FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17196733
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC54700.015.110953145
GCCGACC20850.013.910036145
GTATTAG121350.013.3853371
TATACTG171650.013.3450935
GTATATA318350.013.0518141
GTCCTAG79150.013.0094381
GTCCTAA89650.012.6990271
CCTATAC70250.012.6918743
GTATAAG110600.012.5227381
CTATACT120200.012.4231644
TCTATAC119150.012.2283823
GTATAGA116750.012.1115241
GTCCTAT73600.011.7243891
GTGCTAG95850.011.5749851
GTCTTAG97350.011.54560851
CTTAGAC85100.011.4992943
CGTACGA4500.005170712711.2762295
CTTACAC116750.011.1759013
GTGTATA215950.011.1146521
GTCTTAC85600.011.0973331