FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14572861
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG105050.014.9792661
GTATATA233300.014.8262221
GTATAGG63600.014.4801641
TATACTG125050.013.7969465
GTATAAG100400.013.2173451
GTATAGA99500.013.1182621
CCGACGC26450.012.884328145
GTGTATA160100.012.818051
TATACAG134050.012.5461615
CTAGGAC69000.011.97663
ATACTGT165650.011.8594096
TATATAC233050.011.75764753
GTACTAG46600.011.6708421
GTATTAT161750.011.611341
CTAGTAC45200.011.54707053
GTATTAA145850.011.4353181
GTATATG145950.011.3281141
TTATACT154050.011.1993944
CCTATAC58950.011.0671673
TAGGACT90250.011.0040514