FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21474227
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC32350.014.344209145
GTATATA326150.014.3182171
GTATTAG154900.014.18442251
TATACTG180050.013.7293255
CCTATAC83900.013.2192343
GTATAGA143400.012.5913031
GTATAAG144100.012.5804591
GTATAGG90750.012.4651771
GGATCGT41300.012.4606478
GTGTATA249400.012.35699751
CTATACT142200.012.2347344
GTGCTAG119850.012.1007531
TCTATAC137050.012.0066993
GTATTAA209050.011.7936751
GATCGTT46750.011.6277799
CTTAGAC110500.011.61148553
GTATTAT229800.011.4545251
TACACTG198500.011.3941535
GTCCTAG103750.011.3226321
GTCCTAA115600.011.2283431