FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14701987
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG2901.8697004E-417.4973854
GCCGACC16500.016.258799145
CCTATAC57400.014.396563
CTATACT100100.013.2522674
GTATTAA150000.013.2460431
GTATAGG57500.013.2418381
GTATATA262150.013.0562651
GTATTAG103300.012.9866831
CGTACGG3350.00977902812.9832685
TATACAG139550.012.8824795
GGATCGT22100.012.7901628
CCGTATA19450.012.674475145
TATACTG132450.012.4784455
TGACGCG9309.458745E-1012.474056145
GTATTAT168050.012.211661
TCTATAC92400.012.1597733
GTCCTAG70050.012.1116841
GTATAAG94100.011.8674631
GTATACG14750.011.7990241
GTCCTAT65300.011.5490691