FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21835728
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA306950.015.3550271
GTATTAG153450.014.3651961
GTATAGG90650.013.8383141
GTATACG25000.013.6320991
TATACTG173800.013.4717595
GTATAAG143900.012.8494421
GTGTATA240350.012.6408071
CCGACGC42150.012.384973145
GTATAGA150950.012.3453931
TATACAG200300.012.1237075
GTATTAT219650.011.719311
GTATTAA205550.011.5707441
GTATATG213550.011.5107871
TAGACAG418100.011.4948945
TATATAC309300.011.1085933
CTAATAC156400.011.0306433
CTTACAC155100.010.9361553
ATACTGT240050.010.9315676
TCTATAC144950.010.8018013
GGATCGT45200.010.7440048
Produced by FastQC (version 0.11.5)