FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12133769
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG87100.015.8177911
TATACTG107150.014.6129435
GTATAGG53300.014.420791
GTATATA195950.013.5439771
GTCTAAG66450.013.5312241
TATACAG109700.013.4803025
GTATAAG73450.013.2288891
GGATCGT24400.013.0705068
ACTGATC92000.012.9994718
CCTATAC51150.012.8956493
GTCTTAG70300.012.4807421
GTGTATA136600.012.2622921
GTCCTAG59150.012.2590171
TACACAG152950.012.2277445
GTATTAA122600.012.1839241
TCTATAC79850.012.073253
CTGTCGC41550.012.0365579
GTATAGA81700.011.98181
TACACTG113300.011.8363525
GTGTAGG64100.011.7648321
Produced by FastQC (version 0.11.5)