FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15779514
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC18300.018.620981145
CCGACGC39450.018.010878145
GTCCTAG72500.015.3039731
GTATAGG62300.014.4339241
GTATATA270950.014.1852661
TATACTG141300.014.107635
CTATACT107000.013.8876294
CCTATAC63350.013.6160623
GTATTAG106500.013.4823731
GTATAGA109950.013.3231511
TATACAG144800.013.1158465
GTATAAG101150.013.048371
TCTATAC106100.012.4338933
CCGTACC19450.012.2974929
TATTCCG35300.011.9101395
GTGTATA188500.011.8107591
CTATACA128700.011.7713374
GACGACA23700.011.624948145
GTCCTAT66000.011.53708651
GGATCGT24900.011.3523948