FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23246284
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG169100.015.0512481
TATACTG216650.015.0229935
GTATAGA165300.014.7831171
GTATATA393600.013.78021
GTGTATA266650.013.5152411
GTATAAG154400.013.2907081
TATACCG32150.013.0772325
GTATAGG98050.013.0158971
GGATCGT49600.013.0055748
GTATTAT264150.012.9294271
CCGACGC49450.012.902447145
TACACTG206500.012.4265915
CTATACT159150.012.1609594
GTATATG250800.012.05639651
TCTATAC161250.011.8676473
TATACAG219300.011.8665545
ATACTGT281200.011.7548336
ACTGATC162200.011.7077018
GATCGTT55150.011.6965619
TTATACT260400.011.6080244
Produced by FastQC (version 0.11.5)