FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005866
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC12450.016.306114145
GTATATA246950.013.595731
GTATTAG92600.013.3127731
TATACTG125200.013.2588025
CTATACG10001.6370905E-1113.04799654
CCTATAC49450.013.0465323
GTATAGG51750.013.0317631
GTCCTAC41800.012.3172191
GGATCGT23100.012.2365468
TATACAG118200.012.2041845
GTATAAG80850.011.928951
GTGTATA161750.011.74593451
GTATTAT151250.011.6983611
CTATACT87600.011.5849794
GTATAGA86100.011.370021
GATCGTT24900.011.3515519
GTCCTAT54200.011.1047661
TATATAC249250.010.9932983
GTATAAT149950.010.8325861
GTATATG140550.010.6283771
Produced by FastQC (version 0.11.5)