FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n02_Sample_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n02_Sample_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15786696
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA15200.018.6029145
TGACGCG10150.017.85803145
GCCGACC17400.015.417434145
TATACTG128450.014.72935
GTATTAG103700.014.1956971
GTATATA217900.014.0108431
GTATTAT149050.013.72007751
GTATAGA100600.013.4077031
GTATAGG60400.012.7264811
GTGTATA178250.012.6523241
GTATAAG94150.012.4006651
TAATACT145900.012.3713084
GTATTAA139650.012.0991381
GTCCTAG70600.011.9149641
GGATCGT27600.011.8171218
CTATACT97700.011.797074
CCGTACG5551.5611398E-411.7549574
CCTATAC56500.011.6751683
GTCTTAG86350.011.58927251
TTATACT151650.011.5198334