FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36799406
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404217210.98434034505883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG378760.10292557439649977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG55050.012.7817921
GTATATA196750.012.0930651
GTGTATA136300.011.9746811
CTATACT78900.011.4827844
GTATTAG83850.011.2465061
TATACAG114000.010.8719755
TACACTG107800.010.6232035
TAGACTG78900.010.1967825
CCTATAC52700.010.1774623
TATACTG108250.010.1103515
TCTATAC81100.010.0095723
GTATTAT138450.09.7453461
TAGACAG189750.09.5875825
TATACGG16006.548362E-119.5127197
GTATAGA84800.09.4952071
CTAGACT66600.09.4680214
GTCCTAT49500.09.3789031
CTTACAC77400.09.3643453
GCTATAC46550.09.1865123
CCTACAC64450.09.1092043
Produced by FastQC (version 0.11.5)