FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21527982
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA381300.013.23673251
CCTATAC89000.013.1962763
GGATCGT40150.013.1811598
GTATTAG150000.013.1497051
ATGCCGG42200.012.544658145
TATACAG203100.012.3864235
GTCCTAA111500.012.2920781
GTATTAA202950.012.1129241
TATACTG192500.012.0892865
GTATAGG89700.012.0457041
GTATAAG137800.011.9457921
GTCCTAG102600.011.662051
GATCGTT46250.011.599779
TCCGTAC36500.011.5202393
GTGTATA271950.011.51948451
CCGTACC32300.011.4470624
GTATAGA141650.011.3139451
TACACTG209600.011.2413495
TCTATAC139250.011.1415333
CGTACGA5250.001291330611.0473235
Produced by FastQC (version 0.11.5)