FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8831744
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAC18000.017.728037145
GTATAGA53950.015.188361
CCTATAC31200.014.4068013
GTATATA152500.014.3126651
CTCCGAC11150.014.309624145
TATACGG8401.891749E-1013.8093615
CTATACT58300.013.55472854
GTATTAG58650.012.9821061
ATGCCGG68850.012.956318145
CACGAGC35600.012.834265145
TATACTG81600.012.705125
GTCCTAG37700.012.5024711
GTATAAT92300.012.3345081
TATACAG79400.012.2353725
GTAACGC5351.1073526E-412.196053
GTATAAG57200.011.7899181
GTGTATA107250.011.76456451
TCTATAC53650.011.3511533
GTCCTAT37350.011.2605921
ATAATAC92300.011.2321993
Produced by FastQC (version 0.11.5)