FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20177192
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC82250.016.5711213
GTCCTAT94500.013.9657841
TATACTG215100.013.5155565
CCTACAC103200.013.2773693
GTCCTAG99600.013.1778631
GTCCTAC65700.013.1343121
CTATACT145850.012.8245824
GTATATA397150.012.762871
CCGTACC26350.012.6563114
GGATCGT33400.012.5892748
CCTAGAC111800.012.515423
TCTATAC140800.012.4092153
TATACAG197150.012.1719785
GTATTAG140600.012.1201571
GTATAGA137750.012.1077091
CTATACA167050.012.0650264
CCCTATA88050.012.0214752
GTATAGG80050.011.776271
ACTGATC155150.011.7284648
TCCGTAC29700.011.7169543
Produced by FastQC (version 0.11.5)