FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27012729
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG222650.014.522655
GTATATA378550.014.4436411
GTATAGG131150.014.2099791
GTGTATA262400.013.7347621
GTATTAG204450.013.4779881
TATACAG249600.013.0707845
GTATAAG199400.012.6919641
TCTATAC194800.012.393383
CCTATAC122700.012.2900393
GTATAGA200150.012.2821021
TATATAC383050.012.2267653
GTATATG278150.011.8881531
CTATACT182800.011.7791514
ACTGATC190600.011.7150638
TACACCG70950.011.6489335
GTCCTAG112150.011.5739691
CTAATAC199100.011.2517933
TTATACT287950.011.2040914
GTATAAT268400.011.0231631
GTGTAGG149700.010.8990661