FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19757872
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC76050.014.1090943
TATACGG19250.013.5584665
GTATATA359550.013.5123621
GTATAGG76450.013.4687291
TATACTG196250.013.2624455
GTATTAA188100.013.2612791
GTATAGA135150.013.1988111
GTATTAG135750.013.1404731
CTATACT136300.012.7127574
TCTATAC132200.012.6682653
GTCCTAA100600.012.2536841
TATACAG184500.012.0637085
GTGTATA232400.012.0438941
GTATAAG121400.012.00585651
GTCCTAG97950.011.5487751
TATATAC359750.011.5072583
GTATTAT223150.011.5032921
CACGAGC74750.011.448691145
ATGCCGG38850.011.38738145
GTATAAA272300.011.1312611