FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36307690
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA661550.015.60819151
TATACTG348300.015.3200295
GTATAGA251850.013.7623061
GTATTAG270350.013.7592981
GTATAAG238400.013.2612821
GTATAGG159800.013.2044861
CCTATAC155000.013.0966743
GTGTATA437250.012.9516921
TATACAG352400.012.8581775
TCTATAC243850.012.3087023
CTATACT256800.012.2526484
GTATTAT420500.012.2087881
TATATAC660000.012.13816453
ACTGATC315450.011.9735298
GTATTAA366650.011.9648961
CTAATAC259100.011.8640543
GTCCTAG186350.011.7512171
ATACTGT451050.011.556856
TAATACT371100.011.526474
GTATATG390450.011.3471