FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14572861
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA97200.015.2942141
GTATATA230050.014.6893721
GTGTATA153300.014.143881
TATACTG125800.013.4856515
TCTATAC92850.013.1959763
GTATTAG102050.013.0750811
CTTACAC100450.013.0636753
GTATAAG92050.012.8411341
GTATAGG59200.012.7394661
TATATAC232600.012.5300573
ACTGATC106600.012.1053458
GTACTAT78800.011.9634571
TATACAG133100.011.8200255
TACACTG130250.011.4107155
CTATACT96150.011.1596134
GTATTAT156650.011.0638691
CTATACA108150.011.0609934
GTATTAC86600.010.9696551
CTAATAC108500.010.9584923
GTGTAAG104750.010.7996611