FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21474227
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA324000.014.5030241
TATACTG175650.014.1575015
GTATAGG85500.013.8245211
GTATTAG147350.013.5827451
CCTATAC86050.013.14359953
GTCCTAG104650.013.0270841
GTGTATA240550.012.7515691
GTATAAG139450.012.7401981
TCTATAC143550.012.6768443
GTATAGA140900.012.4032261
TATATAC319750.012.1986663
GTATTAT217650.012.1941351
CTAATAC153650.012.1738443
TATACAG194550.011.9622935
GGATCGT38900.011.9274548
GTATTAA209500.011.6647251
ACTGATC168050.011.6046268
CTATACA167000.011.5479744
TCTACAC144950.011.2039313
TATACCG16900.011.1539135