FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14701987
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG136100.014.6494275
GTATATA255400.013.9695321
TATACCG12500.013.92025855
GTACGGA19300.012.7749511
GTGTATA167900.012.5683991
GTATTAG105450.012.4471431
GTATAGA94300.012.227091
TATACAG134000.012.0655235
ACTCGCG12750.011.9407078
GTATAGG55600.011.8687311
TAATACT145300.011.8257534
GTCCTAG70050.011.8014231
GTATAAT157100.011.6322041
TCTATAC98150.011.523513
ATATACT192050.011.4763914
GCTAGAC81550.011.4687543
TATATAC257750.011.4484383
GTATTAT163650.011.3881921
GTCCTAT64700.011.3202171
GTATTAA152250.011.1454141