FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21835728
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG91550.016.396931
GTATATA310300.015.7517461
GTATTAG153600.015.2497211
TATACTG178000.014.1742615
GTATAAG138850.013.6837781
CCTATAC91850.013.0240763
TCTATAC135750.012.7643953
GTATAGA145800.012.2854191
GTGTATA238700.012.0307681
TATACAG189750.011.7299935
TATATAC304200.011.4399523
GTATACG23650.011.3454351
GTATTAT215950.011.2833141
CTAATAC149600.011.2434233
CTATACT143900.011.0841424
GTATTAA192700.010.9888361
GTATATG216600.010.9146481
ACTGATC170350.010.8096078
TCCGTAC32600.010.6749493
GTATAAT204850.010.5848761
Produced by FastQC (version 0.11.5)