FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15779514
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC33300.018.293533145
CCTATAC61600.015.1826523
TATACTG144800.014.5200515
GTATAAG99450.014.0737421
ATGCCGG29950.014.044094145
CACGAGC50750.013.575322145
CTATACT101050.013.3448994
TCTATAC106150.013.3184393
GCCGTCG15250.013.315293145
GTCCTAG75800.013.2028271
TAGCGCG3350.00976999612.9850944
GTATATA272900.012.9414361
TACACTG138050.012.6041495
GTACTAG49600.012.574011
GTATTAG107950.012.4953191
TATACAG141650.012.4885475
GGATCGT27300.012.481158
GTATAGA104900.012.443831
CTTAGAC80250.012.19628053
CTTACAC108350.012.1781453
Produced by FastQC (version 0.11.5)