FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23246284
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG210650.015.6943255
GTATAGA164700.014.0007191
GTATATA393750.013.8856941
GTATAAG159050.013.7230181
GTATAGG101350.013.593981
GTCCTAG102300.013.3968581
GTATTAG168100.013.1998961
GTGTATA269100.013.0960221
TATACCG27000.012.88892655
TCTATAC158300.012.4573953
GTATTAT257350.012.39781951
TACACTG212800.012.3672815
CTATACT155200.012.1923684
GTGTAGG112350.012.069391
TATACAG213100.011.9756325
CCTATAC99150.011.77263
ATACTGT282300.011.7624216
TATAGTG166700.011.4382455
ATATACT304450.011.4066694
GTACGGA38900.011.3709571