FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005866
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG7851.0040822E-913.858199145
TATACTG126250.013.7250015
ATGCCGG30500.013.553772145
GTATATA247600.013.2965741
CCTATAC47150.013.2240493
GTATTAT152850.013.0467321
CTATACT87950.013.0246824
AGACGGC42350.012.501284145
TATACAG120950.012.4681875
GTATTAC71750.012.3302661
GTATAAG81750.012.0638431
TCCGTAC16450.011.8999613
GGATCGT21350.011.8846698
TCTATAC82500.011.6002583
TTCGGCG5651.8347678E-411.552586145
GTATTAG85950.011.4743361
CTATACG10858.8039087E-1011.3596534
GTATAGA87600.011.340991
ACTGATC94100.011.3251688
TATATAC244550.011.26584153