FastQCFastQC Report
Fri 15 Feb 2019
HTJYVBGX9_n01_Sample_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJYVBGX9_n01_Sample_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15786696
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG127800.014.86304955
GTATATA215450.014.3719331
CCTATAC58050.013.6132053
TCTATAC96650.013.2022183
GTGTATA163750.013.063951
CTATACT101000.012.99255854
GTACTAG49750.012.8269491
GTATTAG97650.012.7729151
GTATAAG99300.012.4876481
TATACAG129850.012.4508915
CTCCGAC21100.012.373298145
GTATAGG57900.012.2738641
GTATTAT149200.012.10221
ATAATAC142150.011.9345253
CGAGACG14900.011.681279145
GTATAGA101300.011.6684171
GTATTAA137800.011.6299241
GTATAAT140100.011.2837161
CACGAGC89750.011.231707145
GGATCGT27300.011.15333758
Produced by FastQC (version 0.11.5)