FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n02_dpr200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n02_dpr200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157123
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGTAGACCAATCGAATAGAATTGAAGACCCAGAAATGAAACCACAC2773617.65241244120848No Hit
CAGTAGCATCCATCGACCTAGGTCTGTGAGTTTTAGCTCTCTGCCGCAGC1927512.267459251669074No Hit
GGGCTACCCTGGAAAATACTGAAAAGAGTGGGGAGGGGCTCTAATGATGC133418.490800201116324No Hit
CCGTTCAGGAGGAAGTAAAACCGGCGCTGGGATGTACTGAACCGATTTCA122827.816805941841743No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTTTCT19221.223245482838286No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTTTCTTA14150.9005683445453562No Hit
ACATATAATATATAATTCAAGGCATAGATGAAATATCAAGAGGATTAGAT8130.5174290205762364No Hit
ATTCTGGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTC7710.490698370066763No Hit
GGAAGGCGCTCTGCAAAGTGGGTACGGAAAATTTATCACCTGCTATGTAC7650.4868797057082668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7620.4849703735290187No Hit
CCACAGTGCTTGGAAACGCTGGGCATGGGAATGTCTAATACTGCTTATAT7550.48051526511077314No Hit
CAGCATCATGTGAGGATTAAGTGAATCGCAGTGGGGACTAACTGGTGGGT7540.4798788210510237No Hit
GTCTAAAGGACATATAACTCAATATGTATCCCTGTCCCCAGTGTTGAAAG7490.4766966007522769No Hit
ATCCAGAGCTACATAGTAAGACCCTGTTTAAAAGTAATCTGTAGAGGTCT7360.46842282797553514No Hit
CCTTAAATACCTCTGATGTTGTCTTAGCATGCTAATTCGTTCTTGAACCC7290.4639677195572895No Hit
CCTTGCACCTTGCCTCTGTGTTTTCTCTCACTACAAAGGAAAAAAAAATC7250.461421943318292No Hit
CTGTTACACTTCCTGGGAAGCCTGGAGGGCTGGGTAGGCGTGTCCAACAC7140.45442105866104904No Hit
GTCAAGAGAGATGCCTTTTCATGGATATCTCTCTCTTATGAAGTCCTAAC7080.4506023943025528No Hit
CTTCTGAAGAGAAAGAGAATGGAGTAAAAGCAAGCCAGGAAGAAAATGCA7050.4486930621233047No Hit
GAGCTGAGTGACACCTCGCTAGGAAGAAGCAGCAGCAGGTGTGGCTGAGG6990.44487439776480847No Hit
GGTGACAAACAGGTAGATACCTGGGCTGAATGGCTCATTAGCTCAGCTTA6920.44041928934656294No Hit
GTCCACGGCAGCCATTGTTTTACCAGACTCCGACTTTACTGTACTGTTTC6800.4327819606295705No Hit
GTCATATACTACTTAGTATGAATTAGTTCTGAAAAACAGCTTGTTTTCCA6750.4295997403308236No Hit
GACTTTACCTGTTTGTTCATGTATACACTCGAGTCAACAGAAGCCTCATA6670.4245081878528287No Hit
ATTTAGGGCTGAGCACTCAATAATGAATTATTCTCATCACTTTGCCCAAC6640.4225988556735806No Hit
GAAGAAAGGACGTCAGTCCTTAGAACCTTCGGCTACTAATGTATGCTAGC6580.41878019131508437No Hit
GCTTCCTGAGCTCCCACCCCCAAGCTGAGGCTTCATGATCCTGCTGAGAG6570.41814374725533504No Hit
CTCTTAGCCAGGCTACAGCCCAATCTTGATTTGAAAATCTTGTCACGAGT6530.4155979710163375No Hit
CCTTTAGCCAGTGCTTCCTGCTTATGGGGGAAGTGGCAGTGCTTACTATG6490.41305219477734006No Hit
CCTCAACAGAGGAATGGATACAGAAAATGTGGTACATCTACACAATGGAG6480.4124157507175907No Hit
GTTAGGGTCTTAAGCCTACACCCACAGTGACACACCTACTCCAACAAGGC6450.4105064185383426No Hit
GCTAAGGGCTCCAGGGGGGCCGCCGAGGTGGGCTCGTGTTGCTTTAAGCG6440.4098699744785932No Hit
GGCTCATACTCTGCCTACATACCCTTCAACCTAGATCTCTGGGGTGTCCC6390.4066877541798464No Hit
GACCCAGCCTGTGTATGCTATTTGGTTGGTGGTTCAGTCTCTGAGAGGCT6310.4015962017018514No Hit
GCCTTGAGGTTTCAGGTCTTCCTCAGTATAAATGGTGTACCAGTGATAGA6260.3984139814031046No Hit
CTCCTGAACTCTGAGAAGAACTTTCATATCATTTGCAAATGACTCAATCT6220.39586820516410703No Hit
GGATACACATTAAGGGAACAATTGCAGCGATCTTTTCTCCTTGAGAGGCA6220.39586820516410703No Hit
GTCCAGAGAGGAGCTCTAAGTGCAAAGAACCCAGGGAAAGGAGGTCCCTT6210.3952317611043577No Hit
ATTCTGGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTC6110.38886732050686407No Hit
GTTCAGGTCCTATCACAGTGTCTGAGGATGATGACACCCCATGTCTCAGG6060.3856851002081172No Hit
ATTCTGGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTT6050.3850486561483678No Hit
CTTTGGACATTCTACAGCAACCTGCAAATTTGGTATTCAATAATGCACAA5970.3799571036703729No Hit
ACCTAACCCTAGATACTGAAGTTAGCAGTTAGTTGCTGGCACTGAACTGT5950.37868421555087417No Hit
CTTCCAAGGGCCTGCTATGTATGCAAAGAGGGGCGAAAGCAGAGTCAGAT5940.3780477714911248No Hit
ACGCCCCACAGTCCATGGTGACAGATACATGCAAGTGCTCAATGGATGTC5930.37741132743137545No Hit
CTGTAGGTGAGACCTGATTGCTATGTGTGTGTTATCACACTGGCCGACCC5930.37741132743137545No Hit
GTGTGTGTGTGTATGTATGTGTGGTGTATGGTATGTGTGTGTGCATGTGT5880.3742291071326286No Hit
GTACTGAACTTAGACCCTCCTCAAACATACACCATCACCCCAGTGGCCTG5850.3723197749533805No Hit
AACCAATGCTATCCCTGTGCATTCTTCTATCATTTAGCGATTTAAAGAGC5770.3672282224753855No Hit
GCCCTGGCCAGGGTCAGCTGTGTTAGTGACACAGTGTGGAGGGGGGAGTC5680.36150022593764125No Hit
GTGGATATTAGCCCAGAAACCTTGAATATCCAAGATACAACTTCCAAAAC5650.3595908937583931No Hit
GTCGGGGGCCTTGTGCTACCTCCCTGTTTGGGGTTTTAAGGCTGAACCAT5650.3595908937583931No Hit
GACTGGGGCACTGCAGAAGCTCTCAGGCCAGATATCCTGGTTTATGCTGT5630.3583180056388944No Hit
ACATAGACACATGTGGCTCTGTCTGATTTTTATTTGTTTTAATAGGGGTT5570.3544993412803982No Hit
GTGTGTGTGTGTGTGTGTCTTTGATAGTATGAGTTACTGACCTCCTAACA5530.3519535650414007No Hit
GATAGATACTGAGCCCCAGTTTCCCTGCTCTGCTTCTCCCCCACACCTCA5500.3500442328621526No Hit
ACTTGGAATATGGCAAGAAAACTGGGAATCATGGAAAATGAGAAACATCC5440.3462255685036564No Hit
TACCTAAGGTCCACACCTTCTCCAAAACCACATTCAACAGCCCTGGTACA5310.3379517957269146No Hit
ATTAGGAGGTGTGGCCTTGTCGGAGGAACTGGGTGACTTGGGTGACTTTC5290.33667890760741587No Hit
GTTTATAGTTCATGGTGACAAATTTTCTTCAAAAGCAGAAAAACTGTACA5270.33540601948791715No Hit
TGCCAAACCAGAACAGGTCTTACTGGCACCATAGCCACACAGTGGTCCTC5190.3303144670099222No Hit
GCTCCCTACCTGGGATGAATGGTCGTGTGCACTGTGCCTCCACCCCAAAA5130.32649580265142597No Hit
GTCCTTCAACAGCGGCATAGCACGAGCCCGCTGAGGATGAGTGGGGGACA5130.32649580265142597No Hit
ACACAATCAGATCCTTCTACAAAAGGCTATACTCAACAAAACTGGAAAAC5090.3239500264124285No Hit
GTTGAATAGATTCAGGTTGAATAAGTGTGAAGAATGCATAGGGAGCTGTG4910.31249403333693987No Hit
GGCTATCAGAGCTGGCCGACACTGTGAAAACAACAAGCTGCTTATGTAAC4900.3118575892771905No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTTTCTTACA4890.31122114521744115No Hit
CGTCTGCACGGTTACACTTGTCCCTTGATCAGCGTTGAGCACTTTGATGG4850.30867536897844366No Hit
GTGTTGTGGTTTTGCCTTTCTCCCAATCATAGGACCCGGGAGGCAGAGAC4790.3048567046199474No Hit
GGCTTACTCTGAGAAACCACTTAGTGTGTGTGTGTGTGTATGTGTGATTG4630.29467359966395756No Hit
GTCTAGGTTTAATATGGAAGCTCACACAGTTAAAAAAAAAAGGTGTCAAA4600.2927642674847094No Hit
GGGCTGAATAGGCGACAGGGAGCTGCCACCGACAAAAGTCTTATTAAGGA4570.2908549353054613No Hit
TCTATCTCCAGAGCCAGAGTAACATCTCTACGAAAGGCTGCCTAGGTCAG4410.2806718303494714No Hit
GTGTGTGTGGATCCCTGGATTCTGGTCCTTCCTTTGCCCAGGGACAGGGA4410.2806718303494714No Hit
GGCATGAGGGCCTGAGTTCAGAGCGCCAGCATCCATGGAAAAATCCAGGC4320.2749438338117271No Hit
ACACACAGCATCTGCAGAATCATCAGATAATGCTGAGTATCCACCCTGTC4290.273034501632479No Hit
CTTTAATACAGTTCCTCATGTTGCCGTGACCCCAACCATAAAATTATTTT4240.2698522813337322No Hit
CTCCAGGACCTGGCTGGAGCCCCTGTGGCAGTGTGTGCGAGGTGTGCTCA4210.2679429491544841No Hit
GCATGGCTCTCATCTTCCTGGGGGCTGTGCTTCTCACATCATTGCATCAT4190.2666700610349853No Hit
CAGGTAAGCCCAGAGTGAGCCACGGTGGGTCCCGATGGGCAGCGATTTAG4170.2653971729154866No Hit
GGGCAGGTAGACAGAATGAACAGAACCCTAAAAGAGACATTGACTAAATT4060.25839628825824357No Hit
CCATCACTCAGTAAAGAAGCGGCCCTGGTTCATGAAGCGTTAGTTGCGCG3930.2501225154815018No Hit
GGGTCCCCATACCGCTGACGTCCACCAGGTGGCCAGGAAAGTGTCCCTCA3860.24566740706325618No Hit
GTGTGTGTCTGTGTGTCTGTATGTGTGTGTGTGTGTATGTGTGTGTGTGT3680.23421141398776757No Hit
GCCCGCTCACCTCGCAGTTGGGGAGGTTTCACCAGGGCCACTCTCATCCA3540.2253011971512764No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTTT3440.2189367565537827No Hit
CTGCGGTAGGCGGGAACGAGACGACAGGATCAATGTGGTGCCTGCCAGGC3290.20939009565754219No Hit
CCATCAGCCTGTTTTGTACCTCACAGTCAACAGATGGGTCCAGCGACATT3270.20811720753804347No Hit
ATTCTGGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTAT3250.2068443194185447No Hit
GTCTTAGCTAGTCCTTAAAGAAATCTTTTTTAATGCCTTTTTAAAAAGAA3190.20302565506004852No Hit
CAGTCTGCCTTTGCCTGGCCAAGAAATCCTTTCTCTGAAGTAGATACATA3100.1972976585223042No Hit
GTCCTCATCTGTTTCCCAACTCTGGTTCCTGAGCTGCGGAGGCTGAGTGG3090.1966612144625548No Hit
GGTTGATGGCCACTGCACCACCCACAAAGGCCAGAAAACTAGAAAATCCA2970.1890238857455624No Hit
GGGAAGATATTTGTGAGGTGGAAGAGGACTACCAAAGGGAGGAGGGGGAA2890.18393233326756744No Hit
GCCCTTTAGTGTCCTGTGTTTTACTGAGGATTTGAGCCCTATGCAGGCCG2860.18202300108831934No Hit
CCACCATTCTGAAGACACATACTGACTTGTGCATAGTTGGCAAAGAGTGC2830.18011366890907124No Hit
GCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCG2790.17756789267007375No Hit
ATCTAAACCTCTGCATTCCACTTTATTTTAAATTTATTTATTATTGTTTA2740.17438567237132693No Hit
GCTATGCACACATCACCAGAAACACAAGACCTCTGGACTAACTATCCAAC2570.16356612335558765No Hit
CACTTGGGGATTGTATCTGCTATTGTCCTTAGGAGAGAGGAGCTGGATAA2520.1603839030568408No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTCTTTAT2500.1591110149373421No Hit
GTCTAGAGAGACCCACACTTACGTGGTATAGATCGTTACTGCACACTTGT2450.15592879463859524No Hit
GTGCTAGAGTAAACATATGCAAGAAATGATGCCAGCAGTCCTGGGAGAGA2390.15211013028009904No Hit
GTATAACATATGGTTCTTAGTTGAAATATATAGCTATAAAATGATATACT2210.1406541372046104No Hit
ATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAAC2040.12983458818887114No Hit
CACACAGACACACAGACACACACACACACATACTCACTCACACACACTCA1980.1260159238303749No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGAATTCATCA1940.12347014759137745No Hit
ATTCTGGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTG1930.12283370353162809No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTTTATTTCT1660.10564971391839514No Hit
TCCTGATACCGAGACCAGTCTGCCGTCTTCACAGACACCTGGCAGCGGCT1580.10055816144040021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)