FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n02_dpr199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n02_dpr199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22072
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACATCCACCGTGTAGATC355416.10184849583182No Hit
GCCTATCTTGACCCTGGTGTTTGTTCTGGGGGAGAGAGACGGGGTTTGTA5212.360456687205509No Hit
GAATTGTCCTAAATCCAAGGATTACTGCCTGTGTACACTCACTTCCCAAG4812.1792316056542225No Hit
GTGGTAGGTTAGGTTCAGGGTGAGTGGTAACTGGTACTGAGGAACTGGTT4632.0976803189561433No Hit
GGCAGGAACTGGTGCAGAGGCCATGGAGGAGTGCTGCTTACTGGTTTGCT4452.0161290322580645No Hit
GTCTTAGCCATCCCCACCTCTACCCACCCAACAGCCTTCCTGAAAACCAA4241.9209858644436388No Hit
CCTCTCACTGGATATTTTGAGGGGCAGCGATGAGACCATTGGTTTTGAGG4151.8802102210945995No Hit
CCCCAGGCCTGGGGGTGCTCTTGGCGTTTGGTTTGCCGATGCTGCCCTCC3561.6129032258064515No Hit
GGCCAGATGTGTGTACTTGGCATCGACAGGGGTGTACAGGGTCTGTCTCT3111.409025009061254No Hit
GAACCAGACTTCACAAAACCTCACTGGAGTCGTTTGCTCAATAGCGATAC3051.381841246828561No Hit
CTTTAGGGCTTTAGGGCTTCGTACAGACCATGGACATCTACGTAGTCTTT2971.3455962305183038No Hit
GCCTTGGAGAACGGCGCTCGGCGCGCCTAGCCATTCGGTTGAGTTCCATC2931.327473722363175No Hit
GGTTTAGGCTCCTCAAGTGAGGGCGTAGGGTTTTACTTCTGCCTCCCAGT2931.327473722363175No Hit
ACCCACCTCTATAGGCAGTGATGCTCCACTCTGTGGGAGAAGCCCAACGG2861.2957593330916999No Hit
ATCTCAAACAGACCCAAAAGAAGGAGTAGGAGAAAGAAGACGAGGAAGAG2851.2912287060529177No Hit
GTCCAAATGTCAAAGACGCAGAAGTGGAGTGAGGTATTGAGACTGTCTGA2731.2368611815875317No Hit
TGTGTACACTATAGAGGCAAGAAAAGATGATTTGGGGACTGGAATAAGTG2681.214208046393621No Hit
GTTAAAAGCACTTGCTACTCTTGTAGTAAATTTGAGTTTGTTTTCTAGCA2671.2096774193548387No Hit
GGGGTACACATTGAAACCCTGTCTCAAAAAAACAACAAACAAACAAAACC2591.1734324030445813No Hit
CGTAGTAGCTGGCATGCGTGATCCCAGCACTCCGGCTCTCTGATGCTGGC2551.1553098948894527No Hit
CTGTAACTCCTTCCATGGGTGTTTCACCCTGATCTTTAATTTGTTGTTAC2521.1417180137731062No Hit
CTGTACGTGTCCAGGAGCAGAACCGGATATAAGTGGTACCACTAACAGTA2471.1190648785791952No Hit
ATTCCAGGTTCTACAGTGTGGATTTCTCATTTTTCACGTTTTGCAGTGAT2461.114534251540413No Hit
GAAGGAGCCAGTGCGAGCGATTCTGGAAAAGGGGAGGGGACAGCAACCGG2441.1054729974628488No Hit
GTCCATGGGTGGGTGGGTGGAGGGCAGAGTGGAAGAGTAGGTAAGCGGTT2441.1054729974628488No Hit
ACTTAGGGAAATGGGCGAGGTTGGGAAGAAGGGAACCAGTGTGGTTGGCG2431.1009423704240666No Hit
CCCTTAGCCAGACTAACCAGAGGGGACAGAGACAGTAGCCAAATTATCAA2411.0918811163465023No Hit
GGTATATCATTGGAAATGTAAAAGAGCTAAATACCTAATAAAAAATGGGG2371.0737586081913737No Hit
GATATCACCTGAGTGCAGGGGTGCCTGTCTGTGGCGGGGCTGCTTGTGTC2361.0692279811525915No Hit
GATAAAGATAATTAAGTGTTGAAAACACGGTCATTGATTTAGTGCTGGAA2361.0692279811525915No Hit
GGGGAGGGGGGTAGCTCCCCTACCGGAGCCAGGCAGACCTTGAGCCCATT2321.0511054729974627No Hit
GGCATAGCCTCATACAAGGCAGCTCTAAAAGGGTCCCTTGAGCAAAATCT2311.0465748459586806No Hit
GATCCAGCCTCCCTGCCACGTACTTTGTTTCAGGTCCTAAAAATGGTAGG2271.028452337803552No Hit
CCTTAAATGTGTGCAGCCAGCTTGTGTCCTGAGATATCTCTCTCTCCTGT2241.0148604566872055No Hit
GCTTTAAGTGGAGCTTAATGTGCTATTCTAGTAGGAAGATGGAAAGGAAG2231.0103298296484233No Hit
GTATAACATGTTAATTGCAAATTAAAGCAATCATGAGTTCCACTGCTTCT2160.9786154403769481No Hit
TCCTTCTCCCATTAGGGAGAAGCCCATGAACCTGGGCCTTGCCCATGCTC2130.9650235592606017No Hit
GGACCACCCCAGGGCTTTGCTTGGTTGCAGCCAAGCTTATAGAGATGCAA2120.9604929322218194No Hit
ACACTGTAGAACAGATTAGATGAGTGAGTTACACTGAAAAACACATTCGT2040.9242479159115622No Hit
GGGGTGGGGGGCGTGGAGGCCAGAAGAGGAGGTTGGATCCCCCGAGGCTG2010.9106560347952156No Hit
GCCCTTCTACTGCAGTGCTTAGCCGTGCAACTCTCTCGGGCTGCTGAAGC1960.8880028996013047No Hit
TCCTCTTCCTCCTCCTCCTCCTCCCTGTCTCTTATACACATCTGACGCTG1960.8880028996013047No Hit
GGTCAGAGAGTGGCTTTTGTGAGACCCTGCTTGGCCTTCTCCTTGAGAAA1930.8744110184849583No Hit
CAGCAAGCTTAGGGAAGAGGCTCCCTGCCCCCAAGCTCCAGGGCATGGGA1910.865349764407394No Hit
GGATAAAAGTCCTTGCTATCAAGCCAAGCTGAGGTGATCCCCTGGGAGTC1880.8517578832910475No Hit
GCTCAGTTCAATGGTTGACTGTGAGCATCCATATCTTTATTGGTCCTGTC1800.8155128669807901No Hit
GAGGAAGGTCAGCCCCCTTTTTTTATTTTGTTAATTGGAAGAGAGTCACA1690.7656759695541863No Hit
GGGCATGGTGGCATTCCTCACCTCATGTCAGCTGTCTGATGACATAGTCT1660.7520840884378398No Hit
GAAATACAGGTTGAGCAGTGAAAGCCGTACTGTCTTGGTGCTGTCTCTTA1460.6614715476621964No Hit
CATGATGTCTCTTCACAGCAATAAAACACAAACTAAGATAAACTAAGGAG1400.6342877854295035No Hit
GTCTGTCTGTCAGTCCTCTGAGACAGAGTTTCTCTCTGTAACAGCCCTGT1370.6206959043131569No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTGTCTCTTAT1350.6116346502355926No Hit
GGATATGCAAGGTGCAGTCTGCAGGTGCAATTGTTCTTGACTATGTTTGC1310.5935121420804639No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT1290.5844508880028996No Hit
TCCCAAGCCAGGCGGACACCTGTCGTCTGATCCGGATGGTGGCCGGCTGT1230.5572671257702065No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGATCAGGCTGGCCTTG1210.5482058716926422No Hit
GGCCACGGTTGCGTTCGATTGGGCCACCATGACACCTGTCTCTTATACAC1100.49836897426603843No Hit
GGACAGGGTAGATTCTGTCTGGAAAAGGATTAGTGTAACACCAATTCATC1080.48930772018847407No Hit
GCTCCAGACTCACAAGTGAGCATCACACTGGTTCTGGGGATAAAACGCCG1040.4711852120333454No Hit
GAGATGACTTCTGTTGGTTTCTCCCAGTGTCTGCCCACTTTTGTTTCTCT1040.4711852120333454No Hit
ACTTTATCTGAAAAACATTTATATTCTTGGCAACCAATATAATTATCTCG1030.46665458499456325No Hit
GCTGAGCCACACCCCAGCCTAAATGTTTGTTTTAATCGGCATAGTCCTGT950.4304095686843059No Hit
GGTCAGAGGTGGGAAGAAAGAAAACACTGGCTGGTTCCTAAGGGGGAAGG950.4304095686843059No Hit
CTGTCTCTTTACACATCTGACGCTGCCGACGACATCCACCGTGTAGATCT790.35791953606379123No Hit
TGTTTTAACTGCGGAAAGCCTGGGCATTTTAAGAAAGATTGCAGAGCTCC740.33526640086988035No Hit
GTTTTCGTGATACGCGCGCCAATGGTACGGCCAACATACGGGCTGACTTT670.3035520115984052No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCCTGTC670.3035520115984052No Hit
CTTGCTAAGTCTTCAGAATGGAGGAGTGGAATCACACACCCACCAGGGAC640.2899601304820587No Hit
AACAAGAGACTCAGATTTTATAGACTGACCTCAAGAAGGATACAGTATAT600.27183762232693004No Hit
GCCCTGCTCTGCTCCGTGCCCGCTCTTGGAGCGCGCGGCCGGCGGGCGTA580.2627763682493657No Hit
GTTTAGGGCAGAGAAAGCCAGCTGAATGCTACCCATGCATGCATGCACGC570.2582457412105835No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTGA550.24918448713301922No Hit
GGTGTGTAGTCTCCCCTCCCCCAGCCTGAAACCTGCTTGCTCAGGGGTGG520.2355926060166727No Hit
GATAAATAGTTACAAGTCCTTAAAAAACAGGAAAACACAACCAAACAGGT490.22200072490032619No Hit
CAATTTGGACTTCATTCTTGGTTTCATGTGTTTTGCAAATTGTATGTTGT430.1948169626676332No Hit
GCCCTGGAGAAGAATCCTGGATTTGGATTAGAAAGGGTTATTTAACCACC370.16763320043494018No Hit
GGGTGAGGGTGCCCAGACAATGTCTTCCCTGCCTACCTCCTTCCCCATAA360.16310257339615802No Hit
CACACACACACACACACACACACACACCTGTCTCTTATACACATCTGACG290.13138818412468284No Hit
GGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTGCATTTCCCTGATGAT280.12685755708590069No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA250.11326567596955418No Hit
CTTGTGTCCTCTTTATTAAGGACAAAAATCTCACTGATACTGTACCTTTA240.10873504893077202No Hit
AATGAAACGATTAAACAGTTAAAATATTGTAACCATAATTATAAATAAAG230.10420442189198985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)