FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n02_dpr196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n02_dpr196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687464
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA13540.19695576786566277No Hit
GTATATACCTGTAATAATAATGTTAATTCCTATAAGAATAATGGTAAAGT11150.16219031105628803No Hit
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC10480.15244434617667252No Hit
CTATTAGGGAGAGGATTTGAACCTCTGGGAACAAGGTTTTAAGTCTTACG10210.14851686779234985No Hit
GCATATAAGCTAGTACATTAAATCAATGGTTCAGGTCATAAAATAATCAT9340.1358616596650879No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT9300.13527981101555864No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC9270.13484342452841167No Hit
GGTTAGTGGTAGTAGCATTAGTGAGGGAAGAATAATTTTTAGCATTGTAG8220.11956989747826795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7940.11549695693156296No Hit
GTGTATGTCAGATAACACAGATATGTCCTTATAACATTAGTTTAATGTGT7700.11200586503438724No Hit
ACCATGAGCAATTCAAACAATTAAAACCTCTACTATAATAATTATAGCCT7660.11142401638485798No Hit
CTCCTAGACTTGATCTGGTTAGAAAAAACCATCCCAAAATCCACCTCAAC7240.10531460556480049No Hit
GCATTAGACTGTAAATCTAAACACAGAGGTTTAAATCCTCTTTTTACCAG7010.10196897583000711No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC6980.10153258934286014No Hit
GTACAACAGTACATTTATGTATATCGTACATTAAACTATTTTCCCCAAGC6940.10095074069333085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)