FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n02_dpr194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n02_dpr194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57277
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACGTAGCTTGTGTAGATC2852549.80184018017704No Hit
GATGTGGGACATTCCAGAAATAGTATCAGCCATTACAATGTCCTGGCATA6271.094680238140964No Hit
CTCAGGAGGAGACAGGGAGCTATAGTGGCAGCATGAGTCAGGAGAGAATC6121.0684917156974003No Hit
CCACTATAGAATCACAGTACCACAGAAATGACAAACACAAGCAAGACCAA6031.0527786022312622No Hit
GATCTTGGCTTCTCAAAGCACTCAAGCGAGGCATCAATTCCCCAAGTCCC5991.0457949962463118No Hit
AACCAGTCCTTTGCAGAGACTGAAGGACAACTAGAAACACGTTCACAAAG5981.0440490947500742No Hit
GCAAATACCCTTACCAACTGAGCTATTTATTCTCCCAACCTCCCTATATA5971.0423031932538367No Hit
GGAATAGAAACCCTGACTAAGACACTCACCTCCCACAGAGGAGACCTATT5951.0388113902613616No Hit
GCAGGGACTAGAAGGGTCTCAAGGGAGTTCCTGGTCTGCCTAGATACTTG5831.0178605723065104No Hit
CCTAAGGACAGTGGTGCAAATTCTTAGAGAAGTCCTGTATAAGTGGTATA5831.0178605723065104No Hit
CCACTGTAGCAGGTTTACAGGGAAGTGCCTGCAGTGCCTAGTTATAGGGA5811.0143687693140353No Hit
GGGTGTGTATGTTGGTACAAGTTGAGGTTCATGAGCCCTGTTAAGTCCCC5580.974213034900571No Hit
GTATGTGTGTCTGTGAGCTACATGTGTTTAGCATCTGCAGAGACCAGAAG5580.974213034900571No Hit
GGGCTGCTGCTTTCCTGGGTGGAGGAAGACGGGAGAGCTCTGAGAGGAAG5530.9654835274193829No Hit
GTTTTTAAAACAGCAACAGAAAATTCCTTTATGGAATCTAGCCACAGGCT5000.8729507481187911No Hit
CCTTCAACCTTGTAGCTGATTGTAAGAATGCACTGCTCGAGTTACAACAC4960.8659671421338407No Hit
TCCCTACCTCGTGTAGAAGCACCAGAAGCTTATTTTCAGTTCCCTGGAAA4960.8659671421338407No Hit
GGTGAAGGCTTTAATGCTAACCCAAGACAACCAACCAAAAATAATGAACT4850.8467622256752273No Hit
GTACATACCACATGTGTTATTTTGTGGCTGGGTTACCTTACTAAGGTTGA4750.8293032107128516No Hit
GTGTTACCAAGGACACACTTCTAGTGACCTACTTCCTTCAGGCTGTCTCT4660.8135900972467133No Hit
CATAGACCCTGTCTTCCCACCTCACGCCCACTTCCCACTGTTCCTGAAAA4630.8083523927580006No Hit
CTCTGAGCCTGAGTAAATGCAGAAGAAAAGTCATGTGTGACGCCTGCACA4570.797876983780575No Hit
GAACAAAGGTGCCGAGGATAGACAAGCAAGCCATGCCCCAGGAATTTGTC4510.7874015748031495No Hit
TCTCAAGACAGAACAGGCTGCTGTCCCTGGGTATCTGTTAGCCAGGGTCA4490.7839097718106744No Hit
AGGTTGATCTGAACTTGTGACAATCACCACATACAACACTGCCTGTGGTC4350.7594671508633484No Hit
CCACAGTATGAACTTCAAACAGCAGGTGTGCTCTTTCTCTCTGAGACAGT4220.7367704314122597No Hit
GCCCCAGGCTGCCTGGCAGCTTTGAAGCTTCCAACATAAGTGGACTAACT4000.698360598495033No Hit
GATGGCCCATACCTTTAATCTTAGGATCCAATAGGCAGAGACAGGAGGAT3980.6948687955025578No Hit
CTCTTAACCACTGAGCCAGCTCTCCAGCCATAGACATGATTTTTTTTAAA3930.6861392880213698No Hit
GTGTATGACTGTGGGCCTGAGTGTGCTCTTGAAAGAAATCAAAAGTCCTC3910.6826474850288947No Hit
CCTCAGCTCTGACCCTAACACTATCAGTTAGGGTCAAATTCCATCCCTGT3780.6599507655778061No Hit
GCATTGGTTGTACGCTAATAAACAAAACACCACACTCTTTATGAATGTAC3710.647729455104143No Hit
TCCCCACCCTGGTACATGCTTTCACTGGGGAATCAAGCCTTCACAGGACC3650.6372540461267175No Hit
ATGCAGTCCTGCGTGTACCTCATTTTGTGCACCCCCTGATAGACATTCAG3550.6197950311643418No Hit
CTACCGGAGGATCCAGCAATACCTCTCCTGGGCATATATCCAGAAGATGC3490.6093196221869163No Hit
TCTTTAGACAGAACCTCTGATGTGACTTCTATGCCTGGAATCTGGGGTTT3440.6005901147057283No Hit
ATTCCACGTCCTACAGTGGATATTTCTAAATTTTCCACCTTTTTCAGTTT3360.5866229027358277No Hit
AGCTGCTCCTTGGCGCTGCCCTCGGCGTGTTCATGCTCGTCATCCACCTC3240.5656720847809766No Hit
ATCCTGTGCCCTTAATGAGGGAACGCACAAGGAACGAAGAAGCTAGGATT3220.5621802817885015No Hit
AGCTCAGATTTTCCAGAGGAATTGCTTGAAATGTTTGTGGCACATAAAAA3090.5394835623374129No Hit
ACATATAAGTGCTTGGTTTATATTGCAATACTCTGACTTTATTTTTAACT2980.5202786458787996No Hit
ATCTTAATGCATTCCATTACAAAGTGTCTGAGGCTGCCTGATGGGCTACC2940.5132950398938493No Hit
GGCCCGGGTAGCCTGCTTCCCTATGTACCGCAGTCTCAAGTTCCGCGCGA2910.5080573354051364No Hit
GATTTGGGTTATGGATCAGTCAGTTTCGGCTGAGTTCTACAAATGACCCC2890.5045655324126613No Hit
ACATAGTAGTTAGTCACCTTATCACAAGTCTAGGCCAAGACTGCCTCCTG2880.5028196309164237No Hit
CCTGGAATATGGGGAGAAAACTGAACATCACGGAAAATGAGAAATACACA2850.49758192642771093No Hit
AATTCACATCAGAATGTGAGTACAAAAATCTCACGCCTTTCAGGTTTTCT2760.4818688129615727No Hit
CTGTCCTTATACACATCTGACGCTGCCGACGACGTAGCTTGTGTAGATCT2490.43472947256315797No Hit
CTGTCTCTATACACATCTGACGCTGCCGACGACGTAGCTTGTGTAGATCT2200.3840983291722681No Hit
GTGTTTACCTTCTGCCATTCCTAGGCGATACATCATGAAAGCAAATTATC2200.3840983291722681No Hit
GCATTGCTTAATACGAGTATCTACATAAGAGAAGGCAATCATTTAAACAT2120.37013111720236747No Hit
ACATACAACTTGTAAGTAGCCACTGGGCTTCTCTCTTCAGGGCAGGAATT1920.3352130872776158No Hit
GATGTGCACCTCGCCGGTGTGAAGCGCGGCGGCGATGTCGCCGGCCGACA1780.31077046633028965No Hit
CTGTCTCTTATAACATCTGACGCTGCCGACGACGTAGCTTGTGTAGATCT1740.3037868603453393No Hit
CATCTATTCATGTCCCTAGAAATGCAGCTACAGTCACAGTGAGTCAGTCT1740.3037868603453393No Hit
CACATACACACATACACACACATACACACTGAACAAATAAGCAAAATGTT1650.28807374687920106No Hit
GGAGGTGGCCATATGGCTACGTGAGAGAATCGCGTTCGAGGCAGACAGAG1640.28632784538296346No Hit
TCACAAAAGTGCTGCATAATGGTTATTCAGCTTGCTGTTCTCCCATATTT1560.2723606334130628No Hit
CCGAGCCCACGAGACACCGCTGTCTCTTATACACATCTGACGCTGCCGAC1460.25490161845068704No Hit
GATAAGAACATTGGCTTTGCTAATGTAAGGGACCAAGTACAAATCCCTAG950.16586064214257032No Hit
CTTCTGTCCACTCCTAGTTAATGTGACTTCTCCCTGCAGGGTTTCTGTTA650.11348359725544285No Hit
GGCTACAGCAGTTTGTATTCCTGCTAGCAGTGAGTGAGTTTTCCTCTTTC610.10649999127049252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)