FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59727752
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG950769415.918385811674279RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG59114029.897245086337755RNA PCR Primer, Index 16 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10598451.774459885917019No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG3403420.5698222159775912RNA PCR Primer, Index 16 (96% over 28bp)
GGCTCGGAGATGTGTATAAGAGACAGCCGAGCCCACGAGACACCGTGTAG2200440.3684116556069279No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1183120.19808547289708814RNA PCR Primer, Index 16 (96% over 28bp)
TGTCTACACGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCGA1079020.18065638900991954No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG1068970.17897375411014968RNA PCR Primer, Index 16 (96% over 28bp)
GTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCGAGCCCATCTCG948180.1587503243048558No Hit
CCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG932220.15607819962820635RNA PCR Primer, Index 20 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC793680.13288295196510996RNA PCR Primer, Index 16 (96% over 28bp)
CCGAGCCCACGAGACACCGTGTAGACAGGGACAGCCGAGCCCATCTCGTA711870.11918580160190861No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAG688190.11522114544006277RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT687200.11505539334545857RNA PCR Primer, Index 16 (96% over 28bp)
GTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACACCGTGTAGACAGGGA600180.10048595165610787No Hit
CGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCGAGCCCATCT598300.10017119010271808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)