FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n01_dpr200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n01_dpr200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157123
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGAATGGATCAATTCGCATTCTTCTACATGATAACCACCAGTTGTG2848718.130381930080254No Hit
TCCTCAGGGTGTGCTGCGGCAGAGAGCTAAAACTCACAGACCTAGGTCGA1984212.628323033546968No Hit
GTCATAGTGAGGTACAACATTCATTCCAGCATTTGAAAAGCAGAGGAAGG134318.548080166493765No Hit
GGCCTGGGGCATCATTAGAGCCCCTCCCCACTCTTTTCAGTATTTTCCAG132878.456432221889857No Hit
GCTTTGGCATCGGCAATTGCCTGCGCAGTTGCGTCTTTCAGCACTTCCAG124587.92882009635763No Hit
CACACACACACACACACACACACACACACACACACGCCAGAATCTGTCTC9070.5772547621926771No Hit
GCATGAGCACGAGCAAGCTGCATTCTTCCATGGTTTCTGCTTCAAGTTCA8220.5231570171139808No Hit
ATACCCAGCTCTTTTCATTAGCCTGGAGAATTGTAACAACACACTTCAGC7760.4938805903655098No Hit
CACCAGAAGAGGGTATCATATCTCTTTATAGGTGGTTGTGAGCCACCACG7740.49260770224601114No Hit
GGATGGTAGGGTTTATGTCTTCTACAGACTTATACTCCAAGGTGGTATGC7620.4849703735290187No Hit
ATTCCAAGCAAGTGACCTGCCAGTAAAGTAGGTTATGATATGAAGCCCAA7620.4849703735290187No Hit
CTCTTCACTTCACCAGCGAGCACCTGACATCTTACTAGCTGAGGCTGTAT7530.47924237699127437No Hit
CTTCTGTTCTGAAAGAGAACTTTCTTCTGTTGTGAAGAACCCCCCGTGGG7430.4728779363937807No Hit
GTTCTAGGTAGAACAGTTTTAATCAAGAGTTTGCCATCCTGACCTCACAG7310.46524060767678826No Hit
GAATACCACCTCCTGGAAGCCCACCAGTGCACTTACTCGCTCTACCCTCT7220.4595126111390439No Hit
AGTCACCACTGTGCTGGCAGGACTGGGATGCACTCTGTTGATGGTGGAAG7180.45696683490004647No Hit
CACACACACACACACACACACACACACACACACACACGCCAGAATCTGTC7060.449329506183054No Hit
GCTCACATGTGTCCATGTGTATGTCTACACCAGTAGGACTGAGGTCAGAG6990.44487439776480847No Hit
CTTCACAGCTGTCTGCAACTCCCGTTCTAGGAGATTCCATGCCATCTTCT6990.44487439776480847No Hit
CGCTAGGGGGATATCATTCCATTCACTTTGGCAACTCACAGGAAAAGGAA6900.43914640122706416No Hit
CAGTAATCCTGAGCACAAAGAGTGGAGCTGTTGGATTGCCATGTATGAAG6860.4366006249880667No Hit
GTCTGTCACTGTGTGCCCTTAACTGCACAGCGGAGGAAACGGATCGATCT6850.43596418092831724No Hit
GCTTACTACTGTTCTAAATAATGTATTTTCCAATGGATTTATGTTTATCC6810.4334184046893198No Hit
GGGTACATGTAGTCCCTGAAGGCCCCTCACTCCCCTCCACCTAGGCTAGA6810.4334184046893198No Hit
GGCCCAGAGTCCTCCTGCAAAGCTGCCTCGAGAGGAGTCTGGAAGCTCCA6760.43023618439057304No Hit
GTTCTGTTCTCACTATTACTGCTCCATGATGCCTCTTCACATCCAGGATG6760.43023618439057304No Hit
ACTGAACCCTGGCCTTTTGTATCCTGCCAGGTTTGAAAAGCTGGACATCA6730.42832685221132494No Hit
CAAACAAACAAACAAACAAACAAACATATTCCCAATGTGTTCTGATGGCA6580.41878019131508437No Hit
CCACAGACTCATGTGTTTTAACAAGCCTATGGGGGCCAGGGAGTGGAACG6550.41687085913583627No Hit
AGTCTACAGTGCCGCCTCCATCAAGGGCACTTTGGACACCCCAGAGATAT6500.4136886388370894No Hit
GGTCCATCCTTTCATCTCAGCTCCAAACTTTGTCTCTGTAACTCCTTCCA6470.4117793066578413No Hit
CCTACTGATAACCTGAAGAGAGGGGCTCTCTGGTCTTGGTAAGGGGGGCA6460.4111428625980919No Hit
TAACTACAGTGGTTGGAAGATGGCAGATGATAAATAAGCCTGTTCACAAC6420.4085970863590945No Hit
GTTAGAAACAGTAAGAGAGACCCCGCTTCAAAGCAAGGTGGAAGGGGAGA6400.4073241982395957No Hit
ATCCATGTCCGAGTGCCATTGCCTGTCCCAACTCCACCAGGCTTCAGAGG6390.4066877541798464No Hit
ATATAGATGTGCTGCACAATTAAACAAAAGTTCTTCGGAGATCTCAATAT6370.4054148660603476No Hit
CACACACACACACACACACACACACACACACACGCCAGAATCTGTCTCTT6320.40223264576160084No Hit
CCACCAGCCTTCAGTATTGGAAGGCATTGTGTGTGGAGGTGCATGTCTGT6320.40223264576160084No Hit
CTCCATAGTAGTGTGTCAAGGTGGGGTGTAGGTTGGGGCGGTTCCTGTCA6310.4015962017018514No Hit
CAACAATGGAAGCCTGAGCCTTGTTCTCAAGCACACATCCAGCACTTTCC6280.3996868695226033No Hit
ATATACAACTCACACATACACACATGCACACACACATACCATACACCACA6240.3971410932836058No Hit
CGCAAGCACTCCGTGTGCACTCTGATCCACAGGAGCCCTCCCCTCGAGGA6190.39395887298485904No Hit
GACCAGAGCTTACTCAGCCGTCTTGAGTTTAAGACTATTAAATATTCAGA6150.3914130967458615No Hit
TCATACCTCTTTGCTTTGTATGACTCTGTGTTTTGACAGAATTTGCAATA6140.39077665268611217No Hit
GTGTACACATGTGTGGTATGTGTATGTATGTGGTGTATGTGCAAGTCTCC6130.3901402086263628No Hit
GTCCACCCATTGTCTCTGAAGCTACTGCGCATATCTGTACCCACCCAGGA6100.3882308764471147No Hit
GTTAGGACCTGGGCAGTTCCATTAATAAAACAATGATCTATTTTATCTAG6090.38759443238736535No Hit
GTCTGCATCATAGCCCTAACTGGCTGGGGACTACTGTGGGCTGCTCTCTT6060.3856851002081172No Hit
GGTCTGCATCACACCGCCATCTAAAGTCTCAGGCCAGAGTTCACATGCAG5990.3812299917898716No Hit
TTGTTAGACAGACCTGAGATCATCTCAGAAGTCGTCAACATTTTCCTTAT5890.37486555119237797No Hit
GCATAAGGGATTCTTGGTGCTCAGAAAAGAGATCAGAGCAGAAAAGACAA5890.37486555119237797No Hit
GTGGGGCAGTCTCTGGATGGTCCAGCTCCAAACTTTGTCTCTGCAACTCC5850.3723197749533805No Hit
GTTTACCACAGCATAAACCAGGATATCTGGCCTGAGAGCTTCTGCAGTGC5830.3710468868338817No Hit
CTGCAACCCCTATTAAAACAAATAAAAATCAGACAGAGCCACATGTGTCT5790.3685011105948843No Hit
CTCTGGAGCAGGTGAAGGCTCCCAGGGGTGAAGGGCACAGGGCTGAGAAG5580.3551357853401475No Hit
CCATATTACAGGTCCTACAGTGTGCATTTCTCATTTTTCAAGTTTTTTAG5570.3544993412803982No Hit
CTGCTACCCTGGGCCCAATTCAACCTACAGCATTTAATTCATTCAACTGA5400.3436797922646589No Hit
GGTAAACACACTACCAACATAAGCCCATGGATTTTTGAAATAGGGTCTGT5390.3430433482049095No Hit
TGGTGAAGGTCCCCAAACCACCTCTGTTTTAAAGAGCCAGATTTTAAGAG5360.3411340160256614No Hit
GTGCCAATCTCTCTCTTACAATCTGGGTAAGCCTTAGCCATGATTTCTTT5340.3398611279061627No Hit
GGATGGATGATTGCTTTAATGTGTTCTTGGATTCGGTTAGCGAGAATTTT5330.3392246838464133No Hit
TCACAGACCCATGTTCCACAGCCTTGACCTAGAAACACAACCTGTTTCCT5280.3360424635476665No Hit
GTCCTAGAGTCTAGGCATCCCCTCTCCATGGGTGTCCTGGGGCTGTCCCC5110.3252229145319272No Hit
CTATAATTCATGTGTATGAAAGTGTGCGCATGGCTAAGCGGAAAGCAGGG5020.3194949179941829No Hit
TATCTGCCCGAGCCCTGTAGAGTAACAGTACTTTAAACCTCTCTCCTGAG5010.3188584739344335No Hit
GGAGGGAGCAGGCCCTCACATTTATTTGTATGTGGAGTTACCCTATGAGT4950.3150398095759373No Hit
CCCTTGTCCCTTCTAAAAATAGCCGGTTTCTGCTCTAGGACACCATGATC4940.31440336551618797No Hit
TGACGAGCCTGTTGTGGTGGCTTATTCCTGTACTTACAGCACTGAGACAG4720.30040159620170187No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCCG4680.2978558199627044No Hit
GGGTAGGATAGCCCTAAGTAGAGGAAAAACAGTTTTGGTCACCCTTGTAC4590.29212782342496No Hit
GGTGGATACTCAGCATTATCTGATGATTCTGCAGATGCTGTGTGTCTGTC4500.28639982688721577No Hit
GGCCAAGAGCCACAGGATGCTGACCATCCATGCAAAGATGCGCCAAGTCA4460.2838540506482183No Hit
GCCCCAAGGAGACTGAACTGTCCTCTGTTTTGATAGTTGTCTCTGAAATT4440.28258116252871956No Hit
CACATATACACATACACACACATATACATACACACACATACACACACACG4320.2749438338117271No Hit
GGGGTGGAGCTTCCTGCTCATTCGTTCTGCCACGCCCACTGCTGGAACCT4280.27239805757272967No Hit
GTCTAGGACAGTGGATTTCAACCCGTGGGGTCATGACCCCTTTGGGGGCC4280.27239805757272967No Hit
GCTCATTCCAGGGTCATCTATCGCCTGCAAATAGAAGAGGATGGCAGTGA4270.2717616135129803No Hit
CTACAGAACGCTTCTACGCCCTGCACTGAGCTGCTGCCCAGGGCTGCGGA4190.2666700610349853No Hit
GTTTTCATTGGCCTGCGCTCCCGCTGTGCCCGCCCACCAGCCCTGAGGAG4140.2634878407362385No Hit
GCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCG4080.2596691763777423No Hit
CAGTCACAGACACACACACACACAGACAGACACATACACACACACACATA3940.2507589595412511No Hit
CACACACACACACACACACACACACACACACGCCAGAATCTGTCTCTTAT3560.22657408527077513No Hit
GTACCCCTCCACCTATCCAGCCCCATCCTTAGGCCTCCAGAACACTCACC3540.2253011971512764No Hit
CTGCAGGCATGTCCTCAAGAGCTGCGCTAAGCGTCATCATCCCCTTCTAT3540.2253011971512764No Hit
GTTCTGTGTCGTAGGCGACTCGTACTTGCTTGTCAATCTGTATATGTTAA3370.21448164813553713No Hit
GACGCGTACTTAGGGATGCTAGGCATCCTCAGCCTCCATCTTGTATCTTA3340.212572315956289No Hit
GTTGCGCACAGTCAGCTGGATCGAGTAGAGAAGGGTGAGAGTGAGGTTAG3290.20939009565754219No Hit
CTGTTGTTCAGCCTGACCTGGAACTCATGGCTTAGTCCAAGCTCACCTGG3230.20557143129904595No Hit
AAGCCAACACTTTGCTGCTGTCTCTGCTCCTGAGACCGAGCCGGCCTCAA3170.20175276694054975No Hit
GGGTTAGCTCTCTCATGTTTCCCCATGACTGATCCTTAGCACAACGCCTC3020.19220610604430924No Hit
CTCTTGTCCTCCCAGTTAATCTTCCTTGTCCTTTGTCTGCCCTATCAAGA2980.18966032980531175No Hit
GTGCTACCCAGCAACTGTTGGGTGATCCTGAACCCGCAAGGTCACCTGCT2950.18775099762606368No Hit
CAGTAGTGATGAGTCAGCTCCTTCCACCTTCCAGGGGTGCGGACGAGGTC2890.18393233326756744No Hit
TTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA2800.17820433672982314No Hit
GACATGGGCTGATCACCCACTTTGACCCTTGTTTTTTTTTTTTTTTTTTG2790.17756789267007375No Hit
GGTCCTAGCCACGCGTCTTTATTACATTTGCTCGGTGGTTTATTTTTGTC2710.1724763401920788No Hit
CCACTAGGGAGAGCAGATGGGTCCCCCGTGGCACGCGCTCCTGGAAAGGC2650.16865767583358263No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCC2590.1648390114750864No Hit
GTGTGTATGTATGTGTGTATGTGTGTGAGAGTGTGTGTGTGTGTGTGTAT2570.16356612335558765No Hit
AGACTTGTTGAAACGCGGTTAAGTCGGGGAAAAAACGCATGGTGTTGTTG2570.16356612335558765No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG2560.1629296792958383No Hit
GCTGTAGACATGTGCCCTGGCCTAAGTGACTTAAGTGCCAACCAGCATGA2550.16229323523608893No Hit
AGACTTGATAGTAACTTTTAGGATTTTTCAAGTTTAAGACAAGCTTAAGT2480.15783812681784334No Hit
CACACACACACACACACACACACACACACACACACACACGCCAGAATCTG2250.14319991344360788No Hit
GCTCCCAACTCTAGACCCCATCCCTTGGCAGCCACCAGACCACTGTGTCT2130.13556258472661548No Hit
CTTTAGGACGTGAAATATGGCGATGAAAACTGAAAAAGGTGGAAAATTTA1980.1260159238303749No Hit
CTATAAGGGGGAGTCTCGGAAATGTGAGAGGGGGATTGAGACCACCTCAA1670.1062861579781445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)