FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n01_dpr199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n01_dpr199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22072
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGA359816.301196085538237No Hit
CCTCTGCGCGGCCCACACTGTTCTCTGCTACACACGTGTACGTCCCCTCG5412.4510692279811526No Hit
GGCAAGACTGGTCACCTTGTACCTCTGTCAGGAAGCAGGGAGAGGACAGA4892.2154766219644797No Hit
ACACAGACCCTTGAGGCTGTTGTGCCATACTTTCTCAGATGAACTTTTAC4842.192823486770569No Hit
GTCTTTGGCTGATCTTAGCAAGAGCGAAGGATCACCCGATTCCCTAAGAG4662.11127220007249No Hit
GAGCCTCCCTTGAGGAAATGCCTCCATGAAATCCAACTGTAAGGGCATTT4602.084088437839797No Hit
ACTATACAGAGCACAACCGTTGTTCCTTCTTGTGCGGGTCCCTGTATTAC4391.9889452700253714No Hit
GGTCAGCTAAACTAATTTGAAATTCAGCCCTGAAGCTTTCAAACTTGGTC3691.6718013773106197No Hit
GCTCGGAGCTTGTCACGTGTTTGTTGAGTCTCTAGGTCTTTTTATCCTTT3221.4588619064878579No Hit
ACCCTGTACACCCCTGTCGATGCCAAGTACACACATCTGGCCCTGTCTCT3191.4452700253715114No Hit
AGGAAACTGTTATTACACACACACACAACAGTGTAGAAGTGACACTGAGG3081.3954331279449075No Hit
CTCCTACAGAGTGCCACGGTAAGGAAGGCCAAGGGATCCCCGCCCTGTCC3041.3773106197897789No Hit
CCTTGGGACAGAAATATAAGTTGCAAGATTCCGCCTCCGCGTCGGTTCTG3021.3682493657122146No Hit
CATCAGTAGAGACACTATTAAAAAGCCTTGTAGGTGCAGAATCTGTCTGT2831.2821674519753534No Hit
GTCAAAGACTCAACTAGACATGACCTAAAAATTGGCAGTTGACTTCTTGT2801.268575570859007No Hit
AGTTAAAGGTGGTCGTAAACTGCCCAATGTGGGTGCTAGAAAACAAACTC2791.2640449438202246No Hit
GCCAAGGCCCCGTGAACATGCTGCACTTAGGGAGGCTGGTTTCCCATCTG2771.2549836897426603No Hit
AAGCCACCCCTCCCCCTTTCCTTCCTCTGTCCCACACCCTGCTCAGCCAC2661.2051467923160566No Hit
GACCTGCTGTGGCGGAATAGCTGGGCCTGAGTGGAGACAGTTTCCTGGCT2641.196085538238492No Hit
CATTGGTAGCTTGTGACAAATTCGGAATCGAAGACCCTCCCAGACCTGTC2621.1870242841609278No Hit
ACATCCACTTGACCACTTGAAAAATGCCAAAATCACTGCAAAACGTGAAA2591.1734324030445813No Hit
GTGCTACTGTTAGTGGTACCACTTATATCCGGTTCTGCTCCTGGACACGT2581.1689017760057991No Hit
CTCTAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAACAACAAC2551.1553098948894527No Hit
CAACCTATATACCCATCCACTCACCCAACCGCTTACCTACTCTTCCACTC2531.1462486408118884No Hit
GGTTTCTTCTCTGCCCGCAAGAGAAGAAGAGAGGGGGAGAGAGAGAGAGA2501.132656759695542No Hit
GCCCCAATGACTGAGGTAGAGAAGGATTGAGAAAAGTTTACACAAACTGC2491.1281261326567598No Hit
GACTTATAATGACTCCTTCTTTTGCTTTAGGGGTTATGAAACTGTTTAGA2491.1281261326567598No Hit
GGTAGATACTTCATTCCTCCTTAGAATAAGGAACAAAATACCCATCAAGG2451.110003624501631No Hit
CCTCTAACCTCTGGCTAAGAATTCTGGTCTTCCACAAAAATCGCCAACCA2421.0964117433852845No Hit
GGCTTGCCCTGCGAAATTCCAGCCCTGACTCTCGACTCTGAAATCGCCTA2401.0873504893077202No Hit
CTGTGTAGCCCTGGCTGTCCTGGCACTCACTTTGTAGACCAGGCTGGCCT2401.0873504893077202No Hit
GAGAGAGAGATATCTCAGGACACAAGCTGGCTGCACACATTTAAGGCTGT2341.0601667270750272No Hit
GTCTAGAGTTTATTGGAAATTCAACCCAGATTAATTTCCACTCAGCAGGG2341.0601667270750272No Hit
GGAGTATACAGCTCAATGGTGAAGTGCTTGCAGAGCATGGGCAAGGCCCA2291.0375135918811162No Hit
GTCCCGCACCTGCCAGCAGCCTGAGCCTGGGGACTTCTCCAGACCTCATA2261.0239217107647698No Hit
GTTCTGCATAGTTCAAATCACTCTCAGCAACAAAGTCTTCCATCTTCCTT2251.0193910837259876No Hit
GGAGGAGGAGGAGGAGGAAGAGGACTGTCTCTTATACACATCTCCGAGCC2231.0103298296484233No Hit
TGTCTACTGTGCCTTCTGTGTCTTTATAACTCACTTCTAATTACCACTCT2190.9922073214932946No Hit
CTTATATATACTATCATATCATCTGCAAAAAGTGATATTTTGTCTTCTTC2030.9197172888727799No Hit
GCTTGATGCCCTCCCATGCCCTGGAGCTTGGGGGCAGGGAGCCTCTTCCC2030.9197172888727799No Hit
GGATAGAGTGAGTGTGCTTCAGCAGCCCGAGAGAGTTGCACGGCTAAGCA2020.9151866618339978No Hit
CATCTAATCTGTTCTACATGTGGTTTTTATCATTTTCCATGTTTCTCATT2020.9151866618339978No Hit
GCTTCCCAGCGGAGATTGGAGATGTGTGGCATACTTACGGGCTTCAGTAA1960.8880028996013047No Hit
GACCAATAAAGATATGGATGCTCACAGTCAACCATTGAACTGAGCCTGTC1860.8426966292134831No Hit
GGGGTGTGTGGGGGACTTTGGAGATAGCAAATGTGAATGAAGAAAGTACC1760.7973903588256614No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGAATTGACAGGG1750.7928597317868793No Hit
CCCTGGGTCTCTGTCTGTCACATTCCCTATGCTCCCTCACAGCTGAACCT1700.7702065965929684No Hit
CCAGAGGACAGGTGTCCACCTGGCTTGGGAGGCGGCCTCAGCCTCAGCAG1690.7656759695541863No Hit
CACCAAGACAGTACGGCTTTCACTGCTCAACCTGTATTTCCTGTCTCTTA1490.675063428778543No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTCTCTTAT1480.6705328017397607No Hit
GGCTGTTACAGAGAGAAACTCTGTCTCAGAGGACTGACAGACAGACCTGT1470.6660021747009786No Hit
GGTGCCATCTGACTAACTAGAGCCCCCGGGCAGATGGGAGTATACAGGGC1370.6206959043131569No Hit
CTAAAACTGGACTTCAGCAACAACAGAAACAACACAAAGCCTACAAACTA1300.5889815150416817No Hit
ATATATATACTCACACACACACACACACATATCCATAATAAATGTAGCGT1280.5799202609641174No Hit
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT1280.5799202609641174No Hit
GTCTCAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAATCTACAA1190.5391446176150779No Hit
GTGTCATGGTGGCCCAATCGAACGCAACCGTGGCCCTGTCTCTTATACAC1150.5210221094599492No Hit
GAGTGGTGGTGTGTTTGTGTGAGTGGTGGTGTGTGTTTGTGTGAGTGAGG1130.5119608553823849No Hit
GCACAAGGCTATGATTCAATTTCATCACTAGAATGAAAAAGGTTTGGAAA1110.5028996013048206No Hit
GGAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGA1110.5028996013048206No Hit
GGTTAACTGTCATCTGACAGTGAAGATCGCCATTCGCCTGATGAGATGCT1110.5028996013048206No Hit
GAACAACTCATTGAAAGCCCAGATGCACATAGACTGTGTCTGCTTCAGTT1070.4847770931496919No Hit
GACTATGCCGATTAAAACAAACATTTAGGCTGGGGTGTGGCTCAGCCTGT910.41228706052917724No Hit
GATGGTAGCAGCCCAATAAACCCGTACCATCAGACAGCTGCCCTACCCAC870.39416455237404857No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGCTGTC790.35791953606379123No Hit
CTACTAAGCCCTGCAAGGTGAGTGAACCCCGGCCGAGAATAAGGCCCATG760.3443276549474447No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCC720.32620514679231605No Hit
TACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCC700.31714389271475174No Hit
GATAATCACTGACTCTGAAGAGCTGTCCCTGGTGGGTGTGTGATTCCACT650.29449075752084086No Hit
ACATAGTCCTTGGTACGATTCTCCTTAAAAATAGGGCATTGATGGATGAT600.27183762232693004No Hit
CGTTTCATCGCTGGCCCATTATGCAATGTAAGAAAGGACACATCCCTCCA600.27183762232693004No Hit
CCCAGAGTGAAAGAAAGAATCTCGAACTTCAAGCATTCTTTGCTTTTCTT570.2582457412105835No Hit
CTAAAAAGCTGCGGCCAACCTGCCTGCTTCTGTTTCTAGTTCCGATTAAC540.24465386009423704No Hit
CCGTCCTTGTGGTTTTTTTGGGTTTTTTGTTTTTTGTTTTTTGGGTTTTT480.21747009786154403No Hit
CTACAGGAGGATCCCACAATACCTCTCCTGGGCAAATATCCAGAAGATGT450.20387821674519752No Hit
GGCCTGAGATAGTCTTAGAAGGGATATACCCAGGCACGGTGAATTCAAAG400.1812250815512867No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTCCGA330.1495106922798115No Hit
CTGTAGCCCCAGCCTCGGGGGATCCAACCTCCTCTTCTGGCCTCCCCGCC330.1495106922798115No Hit
GTCTCAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAATCTACAACA310.1404494382022472No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA240.10873504893077202No Hit
GGGTTAGGGAGACTGGTTCTTTTCTTAGAGGTTTGGCTGCATGCCTTTAG240.10873504893077202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)