FastQCFastQC Report
Mon 1 Mar 2021
HTJTLBGXH_n01_dpr194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTJTLBGXH_n01_dpr194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57277
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA2964951.76423346194807No Hit
GGTAACAAATTTACAGTCATGAAGTAGCAACAAAAATAATTTTATGGTTG6401.1173769575920527No Hit
TCTTTAAACTTATCATTCAGACAGATATAGTTTTTCATAGCTCAGGGTTT6241.0894425336522513No Hit
GAGCAGGAGTGTGGTGAAGACTCCTGTGGAAATCTGATCGCTGGTTCACT6181.0789671246748258No Hit
GAATGGTTGATTGATTGTCCCCCAAAGTCATGTCACTGGGTAGCAGCAGG6151.0737294201861132No Hit
GTCCTTAAGAAAAGAAGAAGTTGTATATGCTTTAGGATATACATTTTAGC6141.0719835186898756No Hit
GGATTAAGTGACATTCATGAACTTGGTAGATGTATGTGGCGCCCGAGCCA6131.0702376171936379No Hit
GGTTTCAGGTCTGGTCTTGGTCCCAAGGAGTTGACTGATTATCTATCTTG6091.0632540112086875No Hit
CTCTACTCCATCAGCTAGTTGGAGACCTGAATAGAACAAAAGGAATGAAT5951.0388113902613616No Hit
GTATATGACCTTACAAAGTGTGAAATAGTGAGAAAAATGAGTCTGGAGTG5941.0370654887651238No Hit
GCTTAGGGCCTTAAATATGCAAAGTTTTTTCTTTCTTGTGGCTTCACACA5911.0318277842764112No Hit
CCTCAGGGAGGTAACGGAGCCCAAGTCCTTGGCCTGGGGCAAGGGCACCT5680.9916720498629468No Hit
TGTCTGCTGTTGTGTTATCAGAGGCTCACTTACGTTTTAATGCCGTGTGG5670.9899261483667092No Hit
GCTTTGGAAGAGGGAAACCTCCACTGCACAATGACTCCTACCTGTAGGAG5650.986434345374234No Hit
GAGCGGGGACAGACACTTAGGAGGTAAGTTCCTCAGGTCGTGGGTGGGGG5100.890409763081167No Hit
CTATAAATGTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAAT5070.8851720585924542No Hit
ACTTTATTTGGGGAAAACGAAATGGCTTGTGGAGAACGCACTCTGTGTTG5030.878188452607504No Hit
AGTCAGAATGGCTAAGATCAAAAACTTAGGTGACAGCAGAAGTTGGCAAG4930.8607294376451281No Hit
GGGCAGTCATGATAGGTTCCTTTTTGTCAGTGCTCCATAGCCTCGGTAAT4910.8572376346526529No Hit
CCTGAAGGAAGTAGGTCACTAGAAGTGTGTCCTTGGTAACACCTGTCTCT4840.8450163241789899No Hit
CCATGAGGGGAATCAGCTATGATTTTAATTTCTCCCTCATAATCGTTATT4770.8327950137053267No Hit
ACTCCGCCCTGCCCCGCCCCGCCTCGCCTCGCCTCGCCTCGCCGCCTCTC4760.8310491122090891No Hit
TCTCTAGCCCGAGACTTCAGCCTCACTGCACTGCTTCCCTAGTCAGCCTG4730.8258114077203764No Hit
GTGCCACCCACAGTGGGCTAGGCCCGCCTACATCAGTTAGTCATAAAGAT4690.818827801735426No Hit
CTTGTCACCTCTGCTTCCTGGTCACACTCCTCACCACTTTTTGCTCCAAT4480.7821638703144368No Hit
CATCCCTGTTTCCGTTCCAGTCAAAGCTTGCACTTCAGCTCTGTTGCCTA4170.7280409239310718No Hit
TACTTACAAACACGGGGCTTGTTCATTTTTTCTTTTCTTTTCCTTTTCTT4070.710581908968696No Hit
GTACCGTGTTTCAGAAACAAAGGACAAGACACAGAAAAACAAACAAACAA4070.710581908968696No Hit
ACAGGGATGGAATTTGACCCTAACTGATAGTGTTAGGGTCAGAGCTGAGG4060.7088360074724585No Hit
GGTCCAGGCCAGCCAGGCCTATACAAACTAACATCTAAAACATTTTAAAA3970.6931228940063202No Hit
GCTCAGGCATTTCTTGGCAAGAAAAGAGGCTGATGCTCAAAGACAACTAT3770.6582048640815685No Hit
CCTCCACAGCATCTGCCCCTAGTCTGCCCTTCCGTTCTACGCCTTCGTGT3740.6529671595928557No Hit
GATGTATTGTACTTCCTTCTCTGAATCCGAGCAGGGCACAGCACACAGTG3720.6494753566003806No Hit
GGCTATGGCAGTCTCTACATGGTCCATCCTTTCATCTCAGCTCCAAACTC3550.6197950311643418No Hit
CGTACCCACCTAACCGAGGTGGATGACGAGCATGAACACGCCGAGGGCAG3530.6163032281718666No Hit
GGCATGCAACCATGCCCAGCTCATATCATTTATAATACAGCTAGATGTGG3510.6128114251793914No Hit
CTGTAGGACCTGGAATATGTCGAGAAAACTGAAAATCACGGAAAATGAGA3480.6075737206906786No Hit
GGTCAGCAGAGCAGGTACAAGTCAAATGATCCACACCAAGAGTCTGAAAT3270.5709097892696894No Hit
ATAGAAGCTTTCCTAATCTCCCAGTTTGGCACTGCTTTAAGAAACCGTGT3260.5691638877734518No Hit
CACACACACACACACACACACACACAGTACCTTAGCTTGTCGCTTGCCCT3050.5324999563524626No Hit
GCACAGACATAGCCAAACATGAGTAGTTTCCAATAACGAGATCTGTTCTG3030.5290081533599875No Hit
GTAGAGTCCTTATCGAAATCTGTAAAACAGAAGGAAGGTGCATGCCATGC3010.5255163503675122No Hit
GCCTGCCCCAATCCAATCGCGCGGAACTTGAGACTGCGGTACATAGGGAA3010.5255163503675122No Hit
ATACTGCTCCCTTCCATTTTCTCACCTTCCAGACTTTGGCCTATAATTTA2950.5150409413900867No Hit
ATTCAGTGGGGGTCCAGAACATTTCTTTGATCTACCAGTAGGAATTGGAC2900.5063114339088989No Hit
ATTCCACCTTTTTCAGTTTTCCTTGCCATATTTCACGTCCTAAAGTGTGT2870.5010737294201861No Hit
AGTACTGAGACTATTATCTTTATTGTGTTTCTTACTACTTAAATTGACAC2430.4242540635857325No Hit
CTCTTCAAAACTTCTCATATAAGAGTTTTGCTTACTTTTATATTATGACC2170.37886062468355536No Hit
CCTACCTCCTAACTGAGGAGCTGTGGACGGTTGGAGGGAAAGTCAGAATT2150.3753688216910802No Hit
CTGTCTCTTATCACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGAC2070.3614016097211795No Hit
CTCTAACCCTACTAACCCTAACCTAACCCTAACCTAACCCTAACCCTAAC2060.3596557082249419No Hit
GAGATGGACACTGACTTTGCCCACCTCCTAAGCTTAGTTAAACCCCTGGA1930.3369589887738534No Hit
GGCATAGCTCATGTCCTTGGATGTCATGGGAACTCAGGAAGAGATAGGGA1830.31949997381147754No Hit
GTGTTCCAGGTGGAGAGCGCGGCGCTGGGCGAGGCCCGGCCGGTGGCCGC1820.31775407231524No Hit
CCTTTGTAACCGTGGACCAAAATAAATCCTTCCTCCCTTAGGTTGCTTCT1680.29331145136791387No Hit
CTCTGGACTGGATACTTTTTCAAAAAATGCCTTTGAAACATCAACCTGAC1670.29156554987167627No Hit
CTGTCTCTTATACACATCCCGAGCCCACGAGACACCGTGTAGACAGGGAC1550.2706147319168252No Hit
TTCCTATGCTGATATTATTCTGTGACTATTTCTATAATAATCAGATCCTC1530.2671229289243501No Hit
CTGTCTCTTATACACATCTCCGAGCCAACGAGACACCGTGTAGACAGGGA1330.23220489899959842No Hit
CGGTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGG960.1676065436388079No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGAAAGGGA850.1484016271801945No Hit
GGGTTGACTAGAAAATGAATCATAAAACAAGATACTAATATTAAATAGAC840.14665572568395693No Hit
CTGTCTCTTATACACATCTCCGAGCCACGAGACACCGTGTAGACAGGGAC790.137926218202769No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGAA640.11173769575920528No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGG640.11173769575920528No Hit
TGGGGGTTCTGTGTGGGTCCCTCTGAGCTGCAGTGGTGGTATAACCTGTA620.10824589276673009No Hit
CACACACACACACACACACACACAGTACCTTAGCTTGTCGCTTGCCCTAG610.10649999127049252No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)