FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11630046
Sequences flagged as poor quality0
Sequence length129
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484480.41657616831438155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTAC63150.027.4217113
AGTACTC79350.027.1693255
AAGTACT76600.027.0079564
GTACTCT196500.025.6230966
TGTACTC102400.025.5267125
CTCTGCG185350.025.1884279
TACTCTG201250.024.9468337
TTGTACT80450.024.1983324
TATTGTA84550.020.9779052
ATATTGT80850.020.578821
TGCACTC9650.020.2522725
GTACTAT25450.018.0069646
CAAGTAC69600.017.4338533
TATCCTG23900.017.1209555
CTATACT16850.017.0223184
ACTCTGC297000.016.783728
ACTATGC24500.016.746698
GTCTACG32150.016.6816671
AATGTAC50200.016.5190373
ATGTACT68000.016.5131424
Produced by FastQC (version 0.11.5)