FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n02_Columbia-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n02_Columbia-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38401866
Sequences flagged as poor quality0
Sequence length129
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2325980.6056945253649914No Hit
TCCAAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT2145470.5586889970398834No Hit
GATAAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT1695990.44164260142983675No Hit
ACAATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT1038350.2703905065446559No Hit
ATTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT978720.25486261526978926No Hit
CAAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT835570.2175857808576281No Hit
TGTCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT718610.18712892753701083No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGG586920.15283632310992387No Hit
GATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT562700.14652933792331863No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT531000.13827453072202273No Hit
GGCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT499080.1299624346379418No Hit
AACTTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT493860.12860312569186091No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT489110.12736620663172982No Hit
TAGATGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT483230.12583503103729385No Hit
TAAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT406440.1058386069051957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAC819700.036.5224383
GATAAGT802750.035.8286631
AAGTACT1909350.035.8264664
CCAAGTA1074750.035.2267152
TCCAAGT1097400.034.176051
AGTACTC2139400.033.8516585
ATAAGTA908600.032.229972
GCGTACT275000.031.6760482
GGCGTAC277150.031.5029681
ATTGTAC1452950.030.378463
ATATTGT1530750.028.5991341
TTGTACT1946700.027.8948234
TATTGTA1620900.027.4618362
ACTGGAC60100.027.1854383
ATCCTGC197600.027.0118376
TATCCTG209250.027.0049675
AGGTACT387500.026.7574427
CTCTGCG6186800.026.7050089
TGTACTC3078550.026.6471925
GTACTCT6429650.026.5025316
Produced by FastQC (version 0.11.5)