FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n02_Columbia-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n02_Columbia-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28077370
Sequences flagged as poor quality0
Sequence length129
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT990070.3526220582625794No Hit
CTCTTATATTTATTTCTACATCTAGGATCCGATTTGTATCATTATCATTG604440.21527657326879265No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT490100.17455338587624125No Hit
CTTATATTTATTTCTACATCTAGGATCCGATTTGTATCATTATCATTGAT483420.1721742456647471No Hit
ATATTGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGAT331050.11790634236753655No Hit
AGCTTATGCTCTGACCCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAA330820.11782442586324858No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT318150.11331189495312416No Hit
GGTATGCACTCTTATATTTATTTCTACATCTAGGATCCGATTTGTATCAT293810.10464299184717087No Hit
GAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTT289690.10317561794427328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG89450.038.5917177
GGTATGC115250.037.264081
TGCACTC117450.037.1301275
ATAGGTA96500.035.8982165
TAGGTAT102050.033.5847636
TATAGGT121550.033.452164
ATGCACT153400.028.989074
GGGACCT45850.028.9358025
ATATAGG145250.028.3326683
TAGGGCA43450.027.1414185
GCACTCT165550.026.49056
TATGCAC189750.025.4754473
TCCTGCT115350.024.282057
TCTACCC40200.023.9885488
TCTTATA537400.023.9248032
TATCCTG140600.023.8085125
ATCCTGC129250.023.5706866
CTACCCC56700.023.5075349
CTCTTAT575900.023.3002031
AGTACTC346100.023.1772925