FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n02_At_NLP7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n02_At_NLP7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23963394
Sequences flagged as poor quality0
Sequence length129
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATCCTGCTTATTAATTTGCTGTTTTACTCCTATTGTCTCTTTTGG495010.20656923639447733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAC84950.037.8153083
CTGGACA102750.033.5950784
ATCCTGC246800.030.4619316
TATCCTG263200.029.0305775
GTACTGG137600.026.6569391
TCCTGCT287600.025.628847
ATTATCC305700.025.5777453
CCGATCG39550.023.9169259
CCTGCTT315550.023.5531128
GGACAAT155700.022.840796
CCGTGCC36300.021.9972219
ACCGATC50300.021.2477238
TTATCCT403550.020.7757384
CTACCCC72100.019.8496029
TACTGGA167700.019.3045792
TCTACCC75900.018.3702568
ACCTTGC254450.018.225348
GGTATGC111150.018.0704698
CTGCTTA460400.017.717159
CATTAGG231050.017.7101671
Produced by FastQC (version 0.11.5)