FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n02_At_Chl1.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n02_At_Chl1.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070044
Sequences flagged as poor quality0
Sequence length129
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATCCTGCTTATTAATTTGCTGTTTTACTCCTATTGTCTCTTTTGG247780.12993153031005067No Hit
CTCTTATATTTATTTCTACATCTAGGATCCGATTTGTATCATTATCATTG193550.1014942597930031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAC75400.035.9263653
CTGGACA85000.034.3252644
ACCGATC51600.027.9745468
CCGATCG39000.026.7750539
GGACAAT122650.024.6397766
GTACTGG120750.024.3230361
TATCCTG165250.022.3022675
ATCCTGC163150.022.0998956
GACCTTA70700.021.2862117
GGGACCT70100.021.2049165
GAACCGA59500.020.8532436
CCGTGCC33450.020.5668589
GGTATGC81100.019.9195638
AGGTATG86850.019.8741367
TGCACTC84500.019.6266545
ATTATCC202500.019.0493743
ACCTTGC274000.018.9879348
GGACCTT96100.018.1524056
TCCTGCT192950.018.1460387
CTCTGCG381050.017.9092799
Produced by FastQC (version 0.11.5)