FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11630046
Sequences flagged as poor quality0
Sequence length21
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGG716760.6163002278752809No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACATG82350.014.7610251
GGACATT90450.014.674451
CGCAGGT61550.014.6322831
GGACATC62350.014.5648111
AATATGT80800.014.4409371
AATATGG71700.014.3807221
GACATTG26200.014.3375742
CTTAATT38400.014.2752351
GACATAG23500.014.2300432
GCCTGCG49650.014.2124041
CCAACTG17000.014.2018912
GCCTGCT47700.014.1645761
TTGGATC47050.014.1530651
CGCAGGC52600.014.1138851
GACATGT26200.014.0800132
TCCAACA53700.013.9923471
GACATGA23950.013.9626742
CCAACTC14300.013.947132
GACATCT19850.013.93814852
GCCTGCC38900.013.93753051
Produced by FastQC (version 0.11.5)