FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n01_Columbia-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n01_Columbia-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38401866
Sequences flagged as poor quality0
Sequence length21
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACTGTCTCTTATACACA2840180.7395942686743399No Hit
AATATCTGTCTCTTATACACA2800670.7293057061341759No Hit
TTATCCTGTCTCTTATACACA2084240.542744459344762No Hit
ATTGTCTGTCTCTTATACACA1267610.33009073048689874No Hit
AATCTGTCTCTTATACACATC1260570.3282574862377782No Hit
TTGCTGTCTCTTATACACATC1084410.28238471536773757No Hit
GGACACTGTCTCTTATACACA849140.22111946330941315No Hit
CTGTCTCTTATACACATCTCC813820.21192199358229102No Hit
ATCCTGTCTCTTATACACATC693230.18051987369572092No Hit
GCCCTGTCTCTTATACACATC644940.1679449639244093No Hit
GGACTGTCTCTTATACACATC627700.16345559874616508No Hit
AAGTTCTGTCTCTTATACACA608370.15842198918146322No Hit
ATCTACTGTCTCTTATACACA581310.1513754565989059No Hit
TTACTGTCTCTTATACACATC531890.1385062902932894No Hit
ACTAACTGTCTCTTATACACA390250.10162266594024363No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACT788750.014.8099291
GGACATG1065600.014.7735011
GGACATT1162300.014.7732321
GGACATA901100.014.7557481
GGACATC803850.014.73611
AATATGT1409750.014.7151211
AATATGC1143150.014.7122941
TTATCAT1323350.014.7014271
CGCAGGT491650.014.6978391
ACTAAAT581650.014.6857311
AATATGA1136100.014.6735121
CTTAATT553750.014.6724561
CTTAATA456100.014.6214251
CTTAATG597900.014.6140571
AATATGG1275850.014.606711
GCCTGCT466400.014.5896421
TTATCAC1082100.014.5780421
CTTAATC449100.014.530291
TTGGATT769850.014.5235851
TTATCAG1221450.014.5184341
Produced by FastQC (version 0.11.5)