FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n01_Columbia-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n01_Columbia-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28077370
Sequences flagged as poor quality0
Sequence length21
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATCTGTCTCTTATACACA393920.14029804073529678No Hit
TTATCCTGTCTCTTATACACA287340.10233864496567878No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACT890350.014.8864621
ACTAAAT657350.014.8395451
GGACATT1074400.014.8142191
GGACATA838350.014.8085641
CGCAGGT735400.014.8007631
GGACATG1064900.014.7963351
GGACATC804050.014.7471031
CTTAATT427750.014.6783011
CTTAATG529550.014.6712561
TCCAACG903150.014.6555491
CTTAATA360700.014.6450271
CTTAATC376400.014.6320471
AATATGT742100.014.62468051
CCAACGT272300.014.6175722
CCAACTG272500.014.5985852
AATATGC620150.014.5938841
TCCAACC628700.014.5934791
CCAACCT189150.014.5801192
TTATCAT680200.014.575941
CCAACTA194650.014.5727292
Produced by FastQC (version 0.11.5)