FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n01_At_NLP7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n01_At_NLP7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23963394
Sequences flagged as poor quality0
Sequence length21
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGT1150250.014.8956981
GGACATC933450.014.8258551
GGACATT1330450.014.8244841
GGACATG1232400.014.82309151
GGACATA1083550.014.8219631
GCCTGCT657950.014.7917311
GCCTGCG774850.014.7364231
CGCAGGC924400.014.7194441
AAGTTAT517150.014.7154491
GCCTGCC568000.014.7068241
CTTAATT411800.014.6875141
CTTAATG485650.014.6813751
GCCTGCA645800.014.6774151
CGCAGGA962600.014.6527541
CTTAATA389300.014.6481051
AATATGT681200.014.6260421
CGCAGGG1117600.014.61134051
CCTGCGT192600.014.6029762
GACATGT358050.014.600092
CTTAATC339150.014.58461951
Produced by FastQC (version 0.11.5)