FastQCFastQC Report
Fri 11 Oct 2019
HTHTVAFXY_n01_At_Chl1.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHTVAFXY_n01_At_Chl1.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070044
Sequences flagged as poor quality0
Sequence length21
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACT602300.014.898771
CGCAGGT507000.014.8038871
GCCTGCT469000.014.795821
ACTAAAT356700.014.7729231
TTGGATA565700.014.7051191
TTGGATT690350.014.6794311
TTGGATG700350.014.6754691
AATATGT568350.014.6537121
GCCTGCC403700.014.6453891
GCCTGCG523250.014.6437521
AATATGC456000.014.6369561
TTGGATC543050.014.63611
TCCAACG627700.014.6340771
GGACATT409800.014.6195811
CCAACTA148950.014.6175272
GGACATG380200.014.6115011
CTTAATG285000.014.5968191
CCAACTG183400.014.5871742
GGACATA322300.014.5855431
GCCTGCA429450.014.582941
Produced by FastQC (version 0.11.5)