FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20037759
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3142961.5685187150918423No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1335440.6664617535324184No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG996610.497365997864332No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571390.28515663852429807No Hit
GCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG473610.2363587664668489No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG453280.22621292131520299No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG449230.2241917372097349No Hit
CCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328860.16412014936400823No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210550.10507662059414927No Hit
GGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207900.1037541174140282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGG49450.050.854571
AGGTAGG18500.046.5437434
CTGGGGG52350.045.7563061
GGCAGGA11800.041.9682041
GGTAGGA13600.041.062131
AGGAAGG48450.039.0842134
AGGTCGG5400.038.8915634
TCGGGGG53750.038.7489131
AGGCAGG10400.038.3680234
GGGAGGA20050.035.735431
GGGAGGT14250.035.492031
GGGTAGG9250.035.2015382
GGGCGGA4050.034.6887171
GGTTGGG57350.034.295561
GGGGTAG4650.033.989351
GGCAGGG34150.033.8367351
GTAGGTA5050.033.2792
GCGGGGG103900.033.094091
GGGTGGA10550.031.959651
GGTAGGC4600.031.3046821