FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_9_NxW_C3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_9_NxW_C3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142015
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAT13950.011.2923851
CGCCTAT6850.011.2429291
CGGGAAT21250.010.3783661
CGCGTAT6151.1368684E-910.2457741
CGGGCGC3803.37371E-510.13268315
CGGGCAT10550.09.6226111
CGGCAAT21050.09.4791661
GTCGAAT24550.09.4110881
CGGGGTT12800.09.2985741
CCCGACT12900.09.2264911
CGCGAAT11850.09.1577911
CCCGGAC7302.242814E-99.1112531
GCCGAAT10900.08.9924681
CCGGGAT20700.08.962991
GCCACAT24350.08.9133321
GCGGGCG4351.4791805E-48.85153914
GCCGGAT19900.08.7955761
CGGGGAG11950.08.7882171
CCCGTAT9607.2759576E-128.7515981
CCCGCCT9607.2759576E-128.7515981
Produced by FastQC (version 0.11.5)