FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_8_NxW_C3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_8_NxW_C3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6435091
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT7800.012.5652251
CCGGGAT16300.010.7371561
CGGGAAT17550.010.5707451
CGCGGTT10500.010.0008941
GGCGCCC2850.00342467949.82421867
CGCGTAT4804.3597465E-69.4800151
GCCGACT9600.09.1153981
CGCGGGT6301.8473474E-78.88864770
GCCAGAT21150.08.7714691
CGGGGAT12000.08.7507821
GGGAGAT35900.08.6776551
CCCGCGT5351.7209466E-58.5054331
GCCGATT14150.08.41064551
CGGGGCT8808.4946805E-108.353021
GTGTGCG6755.2906216E-78.2960718
GCGGGGT5151.0206955E-48.1560681
GGGAAAT32800.08.1104811
CGGGCAC7857.4254785E-88.0261951
GCCAGCG10051.6552804E-108.00985734
GGAAAAT56950.07.9901791
Produced by FastQC (version 0.11.5)