FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_7_NxW_C3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_7_NxW_C3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5596305
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4252.7284841E-1114.0022981
CGGGGAT8850.012.6574441
GTGTGCG4953.43789E-1012.0198138
GGGCGCG2050.003348664111.95147211
CGGGAAT14200.011.586411
GCGGGCG2500.001151465611.19963666
CGCGGAT8600.010.9901751
CCTCGCG6704.3655746E-1110.44742367
CGGGGAG8250.010.183491
GCGGAAT10850.010.0016421
GCGCAAT5603.000605E-810.0016411
ACCGCTA10350.09.80714649
CGCGGGT6101.0844815E-89.75378170
GCGCGTT3955.15792E-59.7484361
GCCAGAT16300.09.4494041
CGGACAT9350.09.3598251
CCGACAT14700.09.2872391
CCCTCGC7604.783942E-109.21022866
TCGCGGG8504.5474735E-119.0585369
CCGGGAG13150.09.0509151
Produced by FastQC (version 0.11.5)