FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_6_NxW_C2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_6_NxW_C2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9089717
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT10200.013.7288221
CGGGGAT15350.011.8595551
CGCGGAT12700.011.8532691
GCCAGAT26450.011.7797961
GCCGAAT12500.010.6425811
CGCCCAT15500.010.3896181
GCCGGAT21250.010.3789881
GCGCGCG3208.051311E-49.84337664
CGGCAAT20100.09.7536111
GCGGGAT15650.09.3952511
CGCGTAT6857.2868716E-99.1993121
CCCGACT13750.09.1658591
CCCGCCT10400.09.0887431
GCGAAAT22350.09.0849781
CCGGCAT15800.09.0844831
GCGCCCT5058.291399E-69.0120871
CGCCCTT10150.08.9676921
CGGGCCC3150.00780320038.8894322
CCGGGGT15150.08.7810081
GCGAGAT23250.08.7333021
Produced by FastQC (version 0.11.5)