FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_5_NxW_C2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_5_NxW_C2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513464
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT3300.020.1554991
GGCGCGC1850.001549014413.24359938
CGCGGAT5051.8189894E-1213.1709191
CGGGCTC4104.4898115E-811.9511992
CCGCACA2952.8892395E-511.8667521
CCCACGT2701.6708154E-411.6689731
CGGGGAG3651.7224611E-611.5091241
CGGGGAT5501.6189006E-1011.456811
GGGGAAT9150.011.0950891
CCGGGCT7400.010.8805281
ATCGCAC4207.8919584E-710.8328538
GCGGGAT5905.511538E-1010.6800771
GCCGAAT4158.011297E-610.1224821
GGGGACT5653.439709E-89.9134641
GCCGGAT7151.4915713E-109.7921451
GGGCATC5603.2131538E-79.3749852
CCCGAGT5251.3447425E-69.3351781
GCGGAAT6806.4228516E-99.2665371
AGACGCG4201.0108028E-49.1663922
CCGGACT7302.240995E-99.1113891
Produced by FastQC (version 0.11.5)