FastQCFastQC Report
Tue 7 Jan 2020
HTHHWBGXC_n01_4_NxW_C2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHHWBGXC_n01_4_NxW_C2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7694748
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT6800.012.3541511
CGGGGAT11700.012.2661591
GCCGGAT18200.011.5395911
CCGGGAT19950.011.4046251
CGGGCCC2200.00567315811.1365852
CGCGCGG2903.2498172E-410.86186236
GCGGGGT5251.1017619E-810.6677121
CGGGCCT5401.7083948E-810.3713871
GCGCACT3752.9161765E-510.2676731
CGCACAT7603.6379788E-1210.1325711
CGCCCCT4552.202989E-610.0009781
GCGAGAT21150.09.5990491
GCCAGAT21650.09.5390411
CGCCAAT18050.09.50231551
CGGGTAT12900.09.4970541
GCGGGCC2950.00454858279.49245551
GCGGGAT15600.09.4240011
CCCGCCT10100.09.3573521
GCCGGCT9750.09.3342471
GGGGAAT20100.09.2297591
Produced by FastQC (version 0.11.5)